Keap1 Antibody (A-4): sc-515432
- Keap1 Antibody (A-4) is a mouse monoclonal IgG2a κ, cited in 9 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 40-62 near the N-terminus of Keap1 of human origin
- Anti-Keap1 Antibody (A-4) is recommended for detection of Keap1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Keap1 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Keap1 Antibody (A-4) for WB applications. These reagents are now offered in bundles with Keap1 Antibody (A-4) (see ordering information below).
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Keap1 Antibody (A-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Keap1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Keap1 antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Keap1, or kelch-like ECH-associated protein 1, functions as a stress-sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex, essential for regulating Nrf2 (NF-E2-related factor 2). The interaction between Keap1 and Nrf2 maintains cellular homeostasis, as Keap1 negatively regulates Nrf2 under normal conditions, preventing nuclear accumulation. Upon exposure to oxidative stress or electrophiles, Keap1 undergoes conformational changes that activate Nrf2, enabling nuclear translocation and expression of cytoprotective genes. This dynamic regulation supports cellular defense mechanisms against oxidative damage, underscoring Keap1′s role in maintaining cellular integrity and response to environmental stressors. The specific lysine residues targeted by Keap1 for ubiquitination are located in the N-terminal Neh2 domain of Nrf2, while cysteine residues Cys 273 and Cys 288 in Keap1 are crucial for suppressing Nrf2 activity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Keap1 Antibody (A-4) References:
- Epigenetic regulation of Keap1-Nrf2 signaling. | Guo, Y., et al. 2015. Free Radic Biol Med. 88: 337-349. PMID: 26117320
- Contradictory roles of Nrf2/Keap1 signaling pathway in cancer prevention/promotion and chemoresistance. | Jeddi, F., et al. 2017. DNA Repair (Amst). 54: 13-21. PMID: 28415030
- Small molecules inhibiting Keap1-Nrf2 protein-protein interactions: a novel approach to activate Nrf2 function. | Zhuang, C., et al. 2017. Medchemcomm. 8: 286-294. PMID: 30108745
- Essential Role of Keap1-Nrf2 Signaling in Mood Disorders: Overview and Future Perspective. | Hashimoto, K. 2018. Front Pharmacol. 9: 1182. PMID: 30386243
- Challenges and Limitations of Targeting the Keap1-Nrf2 Pathway for Neurotherapeutics: Bach1 De-Repression to the Rescue. | Hushpulian, DM., et al. 2021. Front Aging Neurosci. 13: 673205. PMID: 33897412
- The role of the Nrf2/Keap1 signaling cascade in mechanobiology and bone health. | Priddy, C. and Li, J. 2021. Bone Rep. 15: 101149. PMID: 34869801
- The Keap1 signaling in the regulation of HSP90 pathway. | Bonura, A., et al. 2022. Cell Stress Chaperones. 27: 197-204. PMID: 35362892
- Keap1 moderates the transcription of virus induced genes through G9a-GLP and NFκB p50 recruitment. | Burns, VE. and Kerppola, TK. 2022. Immunology. 167: 105-121. PMID: 35751391
- Discovery of NAFLD-Improving Agents by Promoting the Degradation of Keap1. | Qi, M., et al. 2023. J Med Chem. 66: 9184-9200. PMID: 37386884
- Cellular Modulators of the NRF2/KEAP1 Signaling Pathway in Prostate Cancer. | Tossetta, G., et al. 2023. Front Biosci (Landmark Ed). 28: 143. PMID: 37525922
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Keap1 Antibody (A-4) | sc-515432 | 200 µg/ml | $322.00 | |||
Keap1 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-531364 | 200 µg Ab; 10 µg BP | $361.00 | |||
Keap1 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-525036 | 200 µg Ab, 40 µg BP | $361.00 | |||
Keap1 Antibody (A-4): m-IgG2a BP-HRP Bundle | sc-547773 | 200 µg Ab; 10 µg BP | $361.00 | |||
Keap1 Antibody (A-4) AC | sc-515432 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Keap1 Antibody (A-4) HRP | sc-515432 HRP | 200 µg/ml | $322.00 | |||
Keap1 Antibody (A-4) FITC | sc-515432 FITC | 200 µg/ml | $336.00 | |||
Keap1 Antibody (A-4) PE | sc-515432 PE | 200 µg/ml | $349.00 | |||
Keap1 Antibody (A-4) Alexa Fluor® 488 | sc-515432 AF488 | 200 µg/ml | $364.00 | |||
Keap1 Antibody (A-4) Alexa Fluor® 546 | sc-515432 AF546 | 200 µg/ml | $364.00 | |||
Keap1 Antibody (A-4) Alexa Fluor® 594 | sc-515432 AF594 | 200 µg/ml | $364.00 | |||
Keap1 Antibody (A-4) Alexa Fluor® 647 | sc-515432 AF647 | 200 µg/ml | $364.00 | |||
Keap1 Antibody (A-4) Alexa Fluor® 680 | sc-515432 AF680 | 200 µg/ml | $364.00 | |||
Keap1 Antibody (A-4) Alexa Fluor® 790 | sc-515432 AF790 | 200 µg/ml | $364.00 | |||
Keap1 (A-4) Neutralizing Peptide | sc-515432 P | 100 µg/0.5 ml | $69.00 |