IL-10 Antibody (E-10): sc-8438

IL-10 Antibody (E-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects IL-10 protein of human origin by WB, IP, IF, IHCP, and ELISA. IL-10 Antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. Interleukin-10, or IL-10, is a 178 amino acid protein primarily secreted by TH2 clones and plays a crucial role in immune regulation. One of its key functions is the suppression of pro-inflammatory cytokine production by TH1 cells, which is vital for maintaining immune homeostasis and preventing excessive inflammatory responses that can lead to tissue damage. Additionally, IL-10 inhibits the activation of macrophages and B lymphocytes by downregulating CD25, the IL-2 receptor, thereby modulating the immune response. The importance of IL-10 in immune regulation is underscored by elevated expression in various pathological conditions, including HIV-1 infection, where IL-10 has been implicated in disease progression. Human and murine IL-10 share significant sequence identity, with 81% similarity at the amino acid level, and both forms exist as non-covalently linked homodimers in solution, exerting biological effects through the IL-10 receptor, a glycoprotein that can be induced in macrophages and fibroblasts by lipopolysaccharide stimulation.

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IL-10 Antibody (E-10) References:

1. Human intestinal alphabeta IEL clones in celiac disease show reduced IL-10 synthesis and enhanced IL-2 production.  |  Kolkowski, EC., et al. 2006. Cell Immunol. 244: 1-9. PMID: 17368439
2. IL-10-transduced bone marrow mesenchymal stem cells can attenuate the severity of acute graft-versus-host disease after experimental allogeneic stem cell transplantation.  |  Min, CK., et al. 2007. Bone Marrow Transplant. 39: 637-45. PMID: 17369865
3. The regulation of IL-10 production by immune cells.  |  Saraiva, M. and O'Garra, A. 2010. Nat Rev Immunol. 10: 170-81. PMID: 20154735
4. Regulation and functions of the IL-10 family of cytokines in inflammation and disease.  |  Ouyang, W., et al. 2011. Annu Rev Immunol. 29: 71-109. PMID: 21166540
5. IL-10 constrains sphingolipid metabolism to limit inflammation.  |  York, AG., et al. 2024. Nature. 627: 628-635. PMID: 38383790
6. IL-10 enhances expression of the IL-2 receptor alpha chain on T cells.  |  Cohen, SB., et al. 1994. Immunology. 83: 329-32. PMID: 7835955
7. Lipopolysaccharide-dependent induction of IL-10 receptor expression on murine fibroblasts.  |  Weber-Nordt, RM., et al. 1994. J Immunol. 153: 3734-44. PMID: 7930590
8. Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages.  |  Feng, L., et al. 1993. Biochem Biophys Res Commun. 192: 452-8. PMID: 8484757
9. IL-10 cooperates with TNF-alpha to activate HIV-1 from latently and acutely infected cells of monocyte/macrophage lineage.  |  Finnegan, A., et al. 1996. J Immunol. 156: 841-51. PMID: 8543841
10. Transmission of HIV-1 from productively infected mature Langerhans cells to primary CD4+ T lymphocytes results in altered T cell responses with enhanced production of IFN-gamma and IL-10.  |  Ludewig, B., et al. 1996. Virology. 215: 51-60. PMID: 8553586
11. Macrophages have cell surface IL-10 that regulates macrophage bactericidal activity.  |  Fleming, SD. and Campbell, PA. 1996. J Immunol. 156: 1143-50. PMID: 8557991
12. IL-10 immunosuppression in transplantation.  |  Bromberg, JS. 1995. Curr Opin Immunol. 7: 639-43. PMID: 8573306

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-10 Antibody (E-10) has 71 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-10 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-10 Antibody (E-10) Specifications

Alternate Product Name: Interleukin-10

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 19-178

Epitope Target: IL-10

Epitope Species: human

Product Reactivity/Detection: IL-10

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 71 citations in various scientific publications

Available Conjugates/Forms for IL-10 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin