IL-10 Antibody (E-10) recommended for detection of IL-10 of human origin by WB, IP, IF, IHC(P) and ELISA

IL-10 Antibody (E-10): sc-8438

IL-10 Antibody (E-10) is rated 4.3 out of 5 by 18.
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Datasheets

    • Anti-IL-10 Antibody (E-10) is a mouse monoclonal IgG2b (kappa light chain) IL-10 antibody provided at 200 µg/ml
    • raised against amino acids 19-178 representing mature IL-10 of human origin
    • IL-10 Antibody (E-10) is recommended for detection of IL-10 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-IL-10 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-10 (E-10): sc-8438.
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Este anticuerpo funciona para determinar IL-10 en conejos? Does this antibody work to determine IL-10 in rabbits?

Asked by: Juan Pablo Rodríguez
Muchas gracias por su pregunta. No recomendamos utilizar este anticuerpo en conejo. Si necesita cualquier información adicional, por favor contacte con nuestro servivio Técnico: [email protected], (800) 457-3801
Answered by: Technical Support Europe
Date published: 2017-09-21

What is the recommended secondary to use with this primary?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 3 out of 5 by from Works well for WB Loaded 30ug of mouse neonatal lung lysates and used the antibody at a dilution of 1:500
Date published: 2019-10-22
Rated 5 out of 5 by from Effect is Very Good Knowledge of the sensitivity and specificity of IL-10 Antibody leads to their more rational selection.It with a precise position effect and very powerful signal.
Date published: 2017-10-16
Rated 5 out of 5 by from available I use the antibody in tubular epithelial cells.Its result is good for me .I will continue to use it for my experiment.
Date published: 2017-10-01
Rated 3 out of 5 by from Good antibody Worked okay with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 1 out of 5 by from IHC Assay Review for IL-10 mAB Application: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from 染色好 IL10抗体效果好,免疫组化染色均匀,阳性着色明显。推荐大家购买,物美价廉,用的1:50用的非常不错。
Date published: 2017-07-21
Rated 2 out of 5 by from Incorrect amount sent Hi I ordered this MAb as a free sample and the vial said 10 ug in 50 ul. I used it once for a western blot (15ul) that ended up not working (probably for my own reasons though). Then when I went to try again, there was none left. Not sure if the full 50 ul was supplied, but it was extremely disappointing to have done all the work of making a second gel and running it only to find that I didn't have enough antibody.
Date published: 2017-06-29
Rated 5 out of 5 by from clean band The antibody used for detect IL-10 by WB,we can easily got the band at 56kda,very clean ,no background.
Date published: 2017-06-24
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