ICADL Antibody (C-4): sc-376044
- ICADL Antibody (C-4) is a mouse monoclonal IgG2b κ ICADL antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 303-331 at the C-terminus of ICADL of mouse origin
- ICADL Antibody (C-4) is recommended for detection of ICADL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ICADL Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ICADL Antibody (C-4) for WB and IHC(P) applications. These reagents are now offered in bundles with ICADL Antibody (C-4) (see ordering information below).
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ICADL Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects ICADL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-ICADL antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ICADL (C-4) monoclonal antibody recognizes a member of the CED/ICE family of cysteine proteases, which plays a crucial role in apoptosis by mediating specific substrate proteolysis during programmed cell death. Key targets include poly (ADP-ribose) polymerase (PARP), gelsolin, and DFF-45/ICAD, each contributing uniquely to the apoptotic cascade. CPP32 cleaves PARP into a signature fragment essential for DNA damage response. CPP32-mediated cleavage activates gelsolin to sever actin filaments, enabling cytoskeletal reorganization during apoptosis. CPP32 also activates DFF-45/ICAD through cleavage, highlighting ICADL′s significance in regulating apoptosis and cellular homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ICADL Antibody (C-4) References:
- Functional differences of two forms of the inhibitor of caspase-activated DNase, ICAD-L, and ICAD-S. | Sakahira, H., et al. 1999. J Biol Chem. 274: 15740-4. PMID: 10336474
- Differential localization of ICAD-L and ICAD-S in cells due to removal of a C-terminal NLS from ICAD-L by alternative splicing. | Samejima, K. and Earnshaw, WC. 2000. Exp Cell Res. 255: 314-20. PMID: 10694446
- Expression of ICAD-l and ICAD-S in human brain tumor and its cleavage upon activation of apoptosis by anti-Fas antibody. | Masuoka, J., et al. 2001. Jpn J Cancer Res. 92: 806-12. PMID: 11473733
- Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state. | Korn, C., et al. 2005. J Biol Chem. 280: 6005-15. PMID: 15572351
- Genetic analysis of the short splice variant of the inhibitor of caspase-activated DNase (ICAD-S) in chicken DT40 cells. | Ageichik, AV., et al. 2007. J Biol Chem. 282: 27374-27382. PMID: 17616520
- Human plasma gelsolin binds to fibronectin. | Lind, SE. and Janmey, PA. 1984. J Biol Chem. 259: 13262-6. PMID: 6092370
- Mch3, a novel human apoptotic cysteine protease highly related to CPP32. | Fernandes-Alnemri, T., et al. 1995. Cancer Res. 55: 6045-52. PMID: 8521391
- Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. | Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
- Lamin proteolysis facilitates nuclear events during apoptosis. | Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814
- DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. | Liu, X., et al. 1997. Cell. 89: 175-84. PMID: 9108473
- Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis. | Kothakota, S., et al. 1997. Science. 278: 294-8. PMID: 9323209
- Caspases: intracellular signaling by proteolysis. | Salvesen, GS. and Dixit, VM. 1997. Cell. 91: 443-6. PMID: 9390553
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ICADL Antibody (C-4) | sc-376044 | 200 µg/ml | $322.00 | |||
ICADL Antibody (C-4): m-IgG Fc BP-HRP Bundle | sc-529810 | 200 µg Ab; 10 µg BP | $361.00 | |||
ICADL Antibody (C-4): m-IgGκ BP-HRP Bundle | sc-522830 | 200 µg Ab, 40 µg BP | $361.00 | |||
ICADL Antibody (C-4): m-IgG2b BP-HRP Bundle | sc-550144 | 200 µg Ab; 10 µg BP | $361.00 | |||
ICADL Antibody (C-4) AC | sc-376044 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ICADL Antibody (C-4) HRP | sc-376044 HRP | 200 µg/ml | $322.00 | |||
ICADL Antibody (C-4) FITC | sc-376044 FITC | 200 µg/ml | $336.00 | |||
ICADL Antibody (C-4) PE | sc-376044 PE | 200 µg/ml | $349.00 | |||
ICADL Antibody (C-4) Alexa Fluor® 488 | sc-376044 AF488 | 200 µg/ml | $364.00 | |||
ICADL Antibody (C-4) Alexa Fluor® 546 | sc-376044 AF546 | 200 µg/ml | $364.00 | |||
ICADL Antibody (C-4) Alexa Fluor® 594 | sc-376044 AF594 | 200 µg/ml | $364.00 | |||
ICADL Antibody (C-4) Alexa Fluor® 647 | sc-376044 AF647 | 200 µg/ml | $364.00 | |||
ICADL Antibody (C-4) Alexa Fluor® 680 | sc-376044 AF680 | 200 µg/ml | $364.00 | |||
ICADL Antibody (C-4) Alexa Fluor® 790 | sc-376044 AF790 | 200 µg/ml | $364.00 | |||
ICADL (C-4) Neutralizing Peptide | sc-376044 P | 100 µg/0.5 ml | $69.00 |