IARS2 Antibody (E-2): sc-393641
- IARS2 Antibody (E-2) is a mouse monoclonal IgG1 κ IARS2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 253-272 within an internal region of IARS2 of human origin
- IARS2 Antibody (E-2) is recommended for detection of IARS2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
- Anti-IARS2 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IARS2 Antibody (E-2) for WB applications. These reagents are now offered in bundles with IARS2 Antibody (E-2) (see ordering information below).
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IARS2 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects IARS2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IARS2 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IARS2, also known as isoleucine-tRNA synthetase, plays a crucial role in protein synthesis by catalyzing the attachment of isoleucine to its corresponding tRNA, a process essential for accurate translation of the genetic code into functional proteins. This enzyme is localized within the mitochondrial matrix, where IARS2 ensures proper functioning of mitochondrial protein synthesis, vital for cellular energy production and metabolism. IARS2 belongs to the class I mitochondrial aminoacyl-tRNA synthetase family and features a characteristic Rossman fold, a structural motif that facilitates nucleotide binding. IARS2 is encoded on human chromosome one, the largest chromosome in the human genome, which underscores IARS2′s significance in cellular function and genetic stability.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IARS2 Antibody (E-2) References:
- Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS. | Bonnefond, L., et al. 2005. Biochemistry. 44: 4805-16. PMID: 15779907
- Transcriptional profiling of genes at the human common fragile site FRA1H in tumor-derived cell lines. | Pelliccia, F., et al. 2007. Cancer Genet Cytogenet. 178: 144-50. PMID: 17954271
- The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase. | Salowe, SP., et al. 2009. J Biol Chem. 284: 9656-62. PMID: 19233850
- Lysine acetylation targets protein complexes and co-regulates major cellular functions. | Choudhary, C., et al. 2009. Science. 325: 834-40. PMID: 19608861
- Defining the determinants for dual targeting of amino acyl-tRNA synthetases to mitochondria and chloroplasts. | Berglund, AK., et al. 2009. J Mol Biol. 393: 803-14. PMID: 19733576
- Search for primitive Methanopyrus based on genetic distance between Val- and Ile-tRNA synthetases. | Yu, Z., et al. 2009. J Mol Evol. 69: 386-94. PMID: 19841848
- Isoleucylation properties of native human mitochondrial tRNAIle and tRNAIle transcripts. Implications for cardiomyopathy-related point mutations (4269, 4317) in the tRNAIle gene. | Degoul, F., et al. 1998. Hum Mol Genet. 7: 347-54. PMID: 9466989
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IARS2 Antibody (E-2) | sc-393641 | 200 µg/ml | $322.00 | |||
IARS2 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-530525 | 200 µg Ab; 10 µg BP | $361.00 | |||
IARS2 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-524030 | 200 µg Ab, 40 µg BP | $361.00 | |||
IARS2 Antibody (E-2): m-IgG1 BP-HRP Bundle | sc-544106 | 200 µg Ab; 20 µg BP | $361.00 | |||
IARS2 Antibody (E-2) AC | sc-393641 AC | 500 µg/ml, 25% agarose | $424.00 | |||
IARS2 Antibody (E-2) HRP | sc-393641 HRP | 200 µg/ml | $322.00 | |||
IARS2 Antibody (E-2) FITC | sc-393641 FITC | 200 µg/ml | $336.00 | |||
IARS2 Antibody (E-2) PE | sc-393641 PE | 200 µg/ml | $349.00 | |||
IARS2 Antibody (E-2) Alexa Fluor® 488 | sc-393641 AF488 | 200 µg/ml | $364.00 | |||
IARS2 Antibody (E-2) Alexa Fluor® 546 | sc-393641 AF546 | 200 µg/ml | $364.00 | |||
IARS2 Antibody (E-2) Alexa Fluor® 594 | sc-393641 AF594 | 200 µg/ml | $364.00 | |||
IARS2 Antibody (E-2) Alexa Fluor® 647 | sc-393641 AF647 | 200 µg/ml | $364.00 | |||
IARS2 Antibody (E-2) Alexa Fluor® 680 | sc-393641 AF680 | 200 µg/ml | $364.00 | |||
IARS2 Antibody (E-2) Alexa Fluor® 790 | sc-393641 AF790 | 200 µg/ml | $364.00 | |||
IARS2 (E-2) Neutralizing Peptide | sc-393641 P | 100 µg/0.5 ml | $69.00 |