HSP 27 Antibody (F-4) recommended for detection of HSP 27 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HSP 27 Antibody (F-4): sc-13132

HSP 27 Antibody (F-4) is rated 4.7 out of 5 by 35.
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Datasheets

    • Anti-HSP 27 Antibody (F-4) is a mouse monoclonal IgG2a κ HSP 27 antibody, cited in 98 publications, provided at 200 µg/ml
    • raised against amino acids 32-108 of HSP 27 of human origin
    • Anti-HSP 27 Antibody (F-4) is recommended for detection of HSP 27 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HSP 27 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HSP 27 (F-4): sc-13132.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for HSP 27 Antibody (F-4) for WB and IHC(P) applications. These reagents are now offered in bundles with HSP 27 Antibody (F-4) (see ordering information below).

What is the best secondary to use with this antibody in Western Blot?

Asked by: TinTin
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2018-03-15
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Rated 2 out of 5 by from Funzionamento discreto Anticorpo testato. Riconosce la proteina over-espressa, meno bene quella endogena
Date published: 2020-07-09
Rated 4 out of 5 by from recommended for WB Works very well for whole cell lysates of HEp-2 and RKO cells when the antibody is diluted 1:500 in BSA/PBS-T. For IF (cells were fixed in 2% formaldehyde) we had to dilute the antibody 1:50 to achieve a good cytoplasmic staining.
Date published: 2020-01-09
Rated 5 out of 5 by from SC-13132 The antibody shows sensitive and specific in IHC.
Date published: 2019-11-21
Rated 5 out of 5 by from perfetto ho provato l'anticorpo come campione gratuito. L'ho usato in western blot su campioni di cellule di ratto, diluito 1:1000 in BSA 5%.
Date published: 2019-10-31
Rated 5 out of 5 by from western blotting Worked well with western blotting at 1:600 dilution in HepG2 Cells.
Date published: 2018-12-24
Rated 3 out of 5 by from WB in HeLa, KM-12 and LIM1215 Works fine with HeLa (50 µg) but no signal in other tested cell lines, Tested both 1:1000 and 1:500 dilutions in 5% Milk/TBST giving a little bit of background.
Date published: 2018-11-26
Rated 5 out of 5 by from Very specific and clear band Very specific and clear signal in western blotting (1:1000) of different type of cells: HeLa, C2C12, 18-HBE, HEPG-2
Date published: 2018-07-19
Rated 5 out of 5 by from Detecting mouse HSP27 protein by WB using sc-13132 Western blotting conditions: Species: Mouse (C57BL6) Tissue: Skeletal muscles Protein amount: 10ug SDS-PAGE gel%: 4-20% gradient gel or 16% gel Blocking buffer: 5% skim milk in PBS Primary antibody dilution: 1:1000 (1:500 - 1:2000 works well) in 5% milk-PBS Washing buffer: TBS - TBST - TBS Secondary antibody detection methods: HRP-conjugated in 5% milk-PBS Band detected: below the 30 kDa protein marker, corresponding to the 27 kDa size of this protein Film and Li-cor exposure: 5s and 15s, respectively Precautions: HSP27 is quite abundantly expressed in murine skeletal muscles and sc-13132 detects this protein very well. If you load a very high amount of protein and use a high primary antibody amount, upon using the HRP-substrate you might get a 'burn-through effect' due to the signal generated and bands might begin to appear hollow. Overall, the blot quality generated using this antibody and the above mentioned conditions was excellent!
Date published: 2018-06-08
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