HSP 105 Antibody (B-7) recommended for detection of HSP 105 of mouse, rat and human origin by WB, IP, IF and ELISA

HSP 105 Antibody (B-7): sc-74550

HSP 105 Antibody (B-7) is rated 5.0 out of 5 by 6.
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Datasheets

    • Anti-HSP 105 Antibody (B-7) is a mouse monoclonal IgG1 κ HSP 105 antibody, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 187-512 of HSP 105 of mouse origin
    • Anti-HSP 105 Antibody (B-7) is recommended for detection of HSP 105 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-HSP 105 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HSP 105 (B-7): sc-74550.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HSP 105 Antibody (B-7) for WB applications. This reagent is now offered in a bundle with HSP 105 Antibody (B-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the recommended fixation method for immunofluorescence staining with HSP 105 (B-7): sc-74550 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Ottimo segnale Segnale specifico in WB in cellule C2C12, HePG2, 16-HBE, 293T
Date published: 2018-06-14
Rated 5 out of 5 by from Excellent antibody for Western blotting. This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It very strongly detects an appropriately sized ~110 kDa target protein in immunoblots.
Date published: 2016-10-13
Rated 5 out of 5 by from Western blot analysis of HSP 105 expression in non Western blot analysis of HSP 105 expression in non-transfected and human HSP 105 transfected 293T whole cell lysates supporting specificity of antibody to immunogen. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Positive immunofluorescence staining of methanol Positive immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Signal via western blot analysis of HSP 105 Signal via western blot analysis of HSP 105 expression in KNRK whole cell lysate. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Positive western blot analysis of HSP 105 Positive western blot analysis of HSP 105 expression in untreated HeLa, heat shock-treated HeLa, untreated NIH/3T3 and heat shock-treated NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-01-03
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