HNF-3 alpha Antibody (Q-6): sc-101058

HNF-3α Antibody (Q-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects HNF-3α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HNF-3α antibody (Q-6) is available in a non-conjugated form and plays a crucial role in the regulation of gene expression within the hepatocyte nuclear factor family, which includes HNF-1, HNF-3, HNF-4, and HNF-6. HNF-3α is primarily located in the nucleus, where HNF-3α functions as a transcription factor that regulates the expression of genes essential for liver function and development. This nuclear localization enables HNF-3α to directly influence the transcriptional activity of target genes involved in metabolic processes, thereby impacting overall liver health and function. HNF-3α is implicated in critical developmental processes, such as gastrulation, where HNF-3α contributes to body axis formation. The interaction of HNF-3α with other members of the HNF family underscores HNF-3α′s importance in maintaining cellular homeostasis and regulating metabolic pathways, making HNF-3α a significant focus for research in liver biology and related diseases.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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HNF-3 alpha Antibody (Q-6) References:

1. Hepatic nuclear factor-3 (HNF-3 or Foxa2) regulates glucagon gene transcription by binding to the G1 and G2 promoter elements.  |  Gauthier, BR., et al. 2002. Mol Endocrinol. 16: 170-83. PMID: 11773447
2. Adenovirus-mediated increase in HNF-3beta or HNF-3alpha shows differences in levels of liver glycogen and gene expression.  |  Tan, Y., et al. 2002. Hepatology. 35: 30-9. PMID: 11786957
3. HNF-3beta is a critical factor for the expression of the Jaagsiekte sheep retrovirus long terminal repeat in type II pneumocytes but not in Clara cells.  |  McGee-Estrada, K., et al. 2002. Virology. 292: 87-97. PMID: 11878911
4. Elevated hepatocyte levels of the Forkhead box A2 (HNF-3beta) transcription factor cause postnatal steatosis and mitochondrial damage.  |  Hughes, DE., et al. 2003. Hepatology. 37: 1414-24. PMID: 12774021
5. Transcription factor HNF-6/OC-1 inhibits the stimulation of the HNF-3alpha/Foxa1 gene by TGF-beta in mouse liver.  |  Plumb-Rudewiez, N., et al. 2004. Hepatology. 40: 1266-74. PMID: 15562441
6. More potent transcriptional activators or a transdominant inhibitor of the HNF1 homeoprotein family are generated by alternative RNA processing.  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
7. The HNF-3 gene family of transcription factors in mice: gene structure, cDNA sequence, and mRNA distribution.  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
8. Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes.  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
9. The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression.  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HNF-3α Antibody (Q-6) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-HNF-3α Antibody (Q-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HNF-3α Antibody (Q-6) Specifications

Alternate Product Name: FOXA1

Product Clone Name: Q-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 367-473

Epitope Target: HNF-3α

Epitope Species: human

Product Reactivity/Detection: HNF-3α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for HNF-3α Antibody (Q-6): N/A

The preferred HNF-3α/β antibody is HNF-3α/β Antibody (E-4): sc-377033.