Date published: 2025-12-12

1-800-457-3801

SCBT Portrait Logo
Seach Input

HNF-3α Antibody (A-3): sc-514695

4.8(6)
Write a reviewAsk a question

Datasheets
  • HNF-3α Antibody (A-3) is a mouse monoclonal IgG2a κ HNF-3α antibody, cited in 10 publications, provided at 200 µg/ml
  • raised against amino acids 51-170 of HNF-3α of human origin
  • HNF-3 alpha Antibody (A-3) is recommended for detection of HNF-3α of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-HNF-3 alpha Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-514695 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HNF-3α Antibody (A-3) for WB applications. These reagents are now offered in bundles with HNF-3α Antibody (A-3) (see ordering information below).

QUICK LINKS

SEE ALSO...

HNF-3α Antibody (A-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects HNF-3 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HNF-3α (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HNF-3 alpha protein plays a crucial role in regulating gene expression in the liver and other tissues, and proves essential for proper development of the endoderm during embryogenesis. HNF-3α is involved in transcriptional regulation of genes vital for liver function, including those responsible for glucose metabolism and lipid homeostasis. HNF-3α′s ability to interact with other members of the hepatocyte nuclear factor family, such as HNF-1 and HNF-4, underscores its importance in maintaining metabolic processes and cellular differentiation. HNF-3α has been implicated in regulating genes involved in body axis formation during gastrulation, highlighting a multifaceted role in development and metabolism. This intricate network of interactions among HNF proteins suggests HNF-3α serves as a key player in liver function and critical factor in overall regulation of gene expression across various tissues.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

HNF-3α Antibody (A-3)

sc-514695
200 µg/ml
$316.00

HNF-3α Antibody (A-3): m-IgG Fc BP-HRP Bundle

sc-531195
200 µg Ab; 10 µg BP
$354.00

HNF-3α Antibody (A-3): m-IgGκ BP-HRP Bundle

sc-524816
200 µg Ab, 40 µg BP
$354.00

HNF-3α Antibody (A-3): m-IgG2a BP-HRP Bundle

sc-547723
200 µg Ab; 10 µg BP
$354.00

HNF-3α Antibody (A-3) X

sc-514695 X
200 µg/0.1 ml
$316.00

HNF-3α Antibody (A-3) AC

sc-514695 AC
500 µg/ml, 25% agarose
$416.00

HNF-3α Antibody (A-3) HRP

sc-514695 HRP
200 µg/ml
$316.00

HNF-3α Antibody (A-3) FITC

sc-514695 FITC
200 µg/ml
$330.00

HNF-3α Antibody (A-3) PE

sc-514695 PE
200 µg/ml
$343.00

HNF-3α Antibody (A-3) Alexa Fluor® 488

sc-514695 AF488
200 µg/ml
$357.00

HNF-3α Antibody (A-3) Alexa Fluor® 546

sc-514695 AF546
200 µg/ml
$357.00

HNF-3α Antibody (A-3) Alexa Fluor® 594

sc-514695 AF594
200 µg/ml
$357.00

HNF-3α Antibody (A-3) Alexa Fluor® 647

sc-514695 AF647
200 µg/ml
$357.00

HNF-3α Antibody (A-3) Alexa Fluor® 680

sc-514695 AF680
200 µg/ml
$357.00

HNF-3α Antibody (A-3) Alexa Fluor® 790

sc-514695 AF790
200 µg/ml
$357.00

What part of the hnf3 does this target?

Asked by: Sm1999
Thank you for your question. sc-514695 HNF-3α (A-3) is raised against amino acids 51-170 of HNF-3α of human origin.
Answered by: BlakeJ
Date published: 2025-02-21

How can HNF-3α (A-3): sc-514695 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-514695 HRP), phycoerythrin (sc-514695 PE), fluoroscein (sc-514695 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_11, d_13, h_10CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_514695, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 130ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from guter Antikörper für Western BlotVerwendung von Zelllysaten, 1:500 verdünnter Antikörper hohe Spezifität mit etwas Hindergrund
Date published: 2017-11-27
Rated 5 out of 5 by from Good for ChIPI efficiently immunoprecipitated FOXA1(HNF-3a)-bound enhancers.
Date published: 2017-11-23
Rated 5 out of 5 by from 很好用我大概是9月初定的小样,配送很快,做了IHC,很好用。当然,可能是细胞本身表达量也高,总之比较好染,就是摸浓度的时候,可以从1:200开始,就可以的了
Date published: 2017-09-22
Rated 4 out of 5 by from HNF-3alpha/FOXA1 in neonatal rat brainDetectable immunoreactivity in neonatal (PN2) rat brain, and absence of immunoreactivity in adult rat brain. Note only specific brain regions were examined. Used at 1:50, signal may be stronger with different dilutions/conditions.
Date published: 2017-08-22
Rated 5 out of 5 by from Works very well in Western blotI used this antibody in LNCaP, PC3 cells, it works very well with single specific band.
Date published: 2017-01-31
Rated 5 out of 5 by from This antibody works well in western.I bought this antibody few months ago. It works well in western blotting in spleen tissue and cultured dendritic cells.
Date published: 2017-01-18
  • y_2025, m_11, d_13, h_10
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_514695, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 11ms
  • REVIEWS, PRODUCT
HNF-3α Antibody (A-3) is rated 4.8 out of 5 by 6.
  • y_2025, m_11, d_13, h_10
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_514695, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 114ms
  • REVIEWS, PRODUCT