HNF-3 alpha Antibody (A-3): sc-514695

HNF-3α Antibody (A-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects HNF-3 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HNF-3α (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HNF-3 alpha protein plays a crucial role in regulating gene expression in the liver and other tissues, and proves essential for proper development of the endoderm during embryogenesis. HNF-3α is involved in transcriptional regulation of genes vital for liver function, including those responsible for glucose metabolism and lipid homeostasis. HNF-3α′s ability to interact with other members of the hepatocyte nuclear factor family, such as HNF-1 and HNF-4, underscores its importance in maintaining metabolic processes and cellular differentiation. HNF-3α has been implicated in regulating genes involved in body axis formation during gastrulation, highlighting a multifaceted role in development and metabolism. This intricate network of interactions among HNF proteins suggests HNF-3α serves as a key player in liver function and critical factor in overall regulation of gene expression across various tissues.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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HNF-3 alpha Antibody (A-3) References:

1. Inactivation of the winged helix transcription factor HNF3alpha affects glucose homeostasis and islet glucagon gene expression in vivo.  |  Kaestner, KH., et al. 1999. Genes Dev. 13: 495-504. PMID: 10049364
2. The HNF-3alpha transcription factor is a primary target for retinoic acid action.  |  Jacob, A., et al. 1999. Exp Cell Res. 250: 1-9. PMID: 10388516
3. Adenovirus-mediated increase in HNF-3beta or HNF-3alpha shows differences in levels of liver glycogen and gene expression.  |  Tan, Y., et al. 2002. Hepatology. 35: 30-9. PMID: 11786957
4. The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas.  |  Lin, L., et al. 2002. Cancer Res. 62: 5273-9. PMID: 12234996
5. A comprehensive search for HNF-3alpha-regulated genes in mouse hepatoma cells by 60K cDNA microarray and chromatin immunoprecipitation/PCR analysis.  |  Tomaru, Y., et al. 2003. Biochem Biophys Res Commun. 310: 667-74. PMID: 14521963
6. Transcription factor HNF-6/OC-1 inhibits the stimulation of the HNF-3alpha/Foxa1 gene by TGF-beta in mouse liver.  |  Plumb-Rudewiez, N., et al. 2004. Hepatology. 40: 1266-74. PMID: 15562441
7. Hepatocyte nuclear factor-3 alpha (HNF-3alpha) negatively regulates androgen receptor transactivation in prostate cancer cells.  |  Lee, HJ., et al. 2008. Biochem Biophys Res Commun. 367: 481-6. PMID: 18178153
8. Functional analysis of surfactant protein B (SP-B) promoter. Sp1, Sp3, TTF-1, and HNF-3alpha transcription factors are necessary for lung cell-specific activation of SP-B gene transcription.  |  Margana, RK. and Boggaram, V. 1997. J Biol Chem. 272: 3083-90. PMID: 9006959
9. Chromatin structure and functional analysis of the mouse HNF3alpha gene.  |  Lodmell, AG., et al. 1998. Gene. 214: 167-76. PMID: 9651517
10. The -4 kilobase promoter region of the winged helix transcription factor HNF-3alpha gene elicits transgene expression in mouse embryonic hepatic and intestinal diverticula.  |  Clevidence, DE., et al. 1998. Int J Dev Biol. 42: 741-6. PMID: 9727829

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HNF-3α Antibody (A-3) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-HNF-3α Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HNF-3α Antibody (A-3) Specifications

Alternate Product Name: FOXA1

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 51-170

Epitope Target: HNF-3α

Epitope Species: human

Product Reactivity/Detection: HNF-3α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for HNF-3α Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred HNF-3α antibody is HNF-3α Antibody (Q-6): sc-101058.