HMG-1/HMGB1 Antibody (D-3): sc-518191
- HMG-1/HMGB1 Antibody (D-3) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 78-101 of HMG-1 of human origin
- recommended for detection of HMG-1 of human origin by WB, IP, IF and ELISA
- Anti-HMG-1/HMGB1 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HMG-1/HMGB1 Antibody (D-3) for WB applications. These reagents are now offered in bundles with HMG-1/HMGB1 Antibody (D-3) (see ordering information below).
HMG-1 Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects HMG-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HMG-1 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. High mobility group (HMG) proteins, such as HMG-1, play a crucial role in regulating gene expression and chromatin structure. HMG-1 binds to DNA and induces significant alterations in its architecture, including bending and unwinding of the double helix, which facilitates transcription factor access to target genes. HMG-1 interacts with various transcription factors, including Oct-2 and members of the NFκB family, highlighting its importance in transcriptional activation. Post-translational modifications, such as phosphorylation, enhance HMG-1′s transcriptional activity, emphasizing its role in cellular responses to stress and inflammation. With versatile applications and significant biological functions, anti-HMG-1 antibody (D-3) serves as an invaluable tool for researchers studying gene regulation and chromatin dynamics in human cells.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HMG-1/HMGB1 Antibody (D-3) References:
- Domain-domain interactions in high mobility group 1 protein (HMG1). | Ramstein, J., et al. 1999. Eur J Biochem. 260: 692-700. PMID: 10102997
- HMG-1 stimulates estrogen response element binding by estrogen receptor from stably transfected HeLa cells. | Zhang, CC., et al. 1999. Mol Endocrinol. 13: 632-43. PMID: 10194768
- Mechanism for specificity by HMG-1 in enhanceosome assembly. | Ellwood, KB., et al. 2000. Mol Cell Biol. 20: 4359-70. PMID: 10825199
- The high mobility group (HMG) boxes of the nuclear protein HMG1 induce chemotaxis and cytoskeleton reorganization in rat smooth muscle cells. | Degryse, B., et al. 2001. J Cell Biol. 152: 1197-206. PMID: 11257120
- Differences between HMG1 proteins isolated from normal and tumour cells. | Alexandrova, EA. and Beltchev, BG. 1987. Biochim Biophys Acta. 915: 399-405. PMID: 3651478
- The Expression of High Mobility Group Box-1 (HMG1) in the Peripheral Blood and Its Relation with Systemic Vasculitis Patients. | Qin, Y., et al. 2023. Iran J Public Health. 52: 2390-2401. PMID: 38106822
- Influence of nonhistone chromatin protein HMG-1 on the enzymatic digestion of purified DNA. | Shastri, K., et al. 1982. Nucleic Acids Res. 10: 5059-72. PMID: 6291002
- Differences in the DNA-binding properties of the HMG-box domains of HMG1 and the sex-determining factor SRY. | Teo, SH., et al. 1995. Eur J Biochem. 230: 943-50. PMID: 7601157
- Homology model building of the HMG-1 box structural domain. | Baxevanis, AD., et al. 1995. Nucleic Acids Res. 23: 1019-29. PMID: 7731789
- The DNA-bending protein HMG-1 enhances progesterone receptor binding to its target DNA sequences. | Oñate, SA., et al. 1994. Mol Cell Biol. 14: 3376-91. PMID: 8164686
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HMG-1/HMGB1 Antibody (D-3) | sc-518191 | 200 µg/ml | $316.00 | |||
HMG-1/HMGB1 Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-550940 | 200 µg Ab; 10 µg BP | $354.00 | |||
HMG-1/HMGB1 Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-550939 | 200 µg Ab; 40 µg BP | $354.00 | |||
HMG-1/HMGB1 Antibody (D-3): m-IgG2b BP-HRP Bundle | sc-550941 | 200 µg Ab; 10 µg BP | $354.00 | |||
HMG-1/HMGB1 Antibody (D-3) AC | sc-518191 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HMG-1/HMGB1 Antibody (D-3) HRP | sc-518191 HRP | 200 µg/ml | $316.00 | |||
HMG-1/HMGB1 Antibody (D-3) FITC | sc-518191 FITC | 200 µg/ml | $330.00 | |||
HMG-1/HMGB1 Antibody (D-3) PE | sc-518191 PE | 200 µg/ml | $343.00 | |||
HMG-1/HMGB1 Antibody (D-3) Alexa Fluor® 488 | sc-518191 AF488 | 200 µg/ml | $357.00 | |||
HMG-1/HMGB1 Antibody (D-3) Alexa Fluor® 546 | sc-518191 AF546 | 200 µg/ml | $357.00 | |||
HMG-1/HMGB1 Antibody (D-3) Alexa Fluor® 594 | sc-518191 AF594 | 200 µg/ml | $357.00 | |||
HMG-1/HMGB1 Antibody (D-3) Alexa Fluor® 647 | sc-518191 AF647 | 200 µg/ml | $357.00 | |||
HMG-1/HMGB1 Antibody (D-3) Alexa Fluor® 680 | sc-518191 AF680 | 200 µg/ml | $357.00 | |||
HMG-1/HMGB1 Antibody (D-3) Alexa Fluor® 790 | sc-518191 AF790 | 200 µg/ml | $357.00 |