HDAC2 Antibody (B-2) recommended for detection of HDAC2 of mouse, rat and human origin by WB, IP, IF and ELISA

HDAC2 Antibody (B-2): sc-55541

HDAC2 Antibody (B-2) is rated 4.0 out of 5 by 4.
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Datasheets

    • HDAC2 Antibody (B-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • recommended for detection of HDAC2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See HDAC2 (C-8): sc-9959 for HDAC2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended fixation method for immunofluorescence staining with HDAC2 (B-2): sc-55541 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Publication using the antibody in IP and Publication using the antibody in IP and WB with skeletal samples -SCBT Publication Review -SCBT Publication Review
Date published: 2015-04-15
Rated 4 out of 5 by from Signal via w estern blot analysis of HDAC2 Signal via w estern blot analysis of HDAC2 expression in Jurkat and NIH/3T3 nuclear extracts. -SCBT QC
Date published: 2015-02-26
Rated 4 out of 5 by from Strong nuclear localization via immunofluorescence Strong nuclear localization via immunofluorescence staining of methanol-fixed HeLa cells -SCBT QC
Date published: 2015-02-16
Rated 4 out of 5 by from Publication using antibody in CoIP and WB Publication using antibody in CoIP and WB with LNCaP cells -SCBT Publication Review
Date published: 2013-07-30
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