HDAC2 Antibody (C-8) recommended for detection of HDAC2 of mouse, rat and human origin by WB, IP, IF and IHC(P)

HDAC2 Antibody (C-8): sc-9959

HDAC2 Antibody (C-8) is rated 4.6 out of 5 by 18.
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Datasheets

    • Anti-HDAC2 Antibody (C-8) is a mouse monoclonal IgG2b (kappa light chain) HDAC2 antibody provided at 200 µg/ml
    • raised against amino acids 435-488 mapping at the C-terminus of HDAC2 of human origin
    • Anti-HDAC2 Antibody (C-8) is recommended for detection of HDAC2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-HDAC2 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-9959 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HDAC2 (C-8): sc-9959.
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I am using HDAC2 (C-8): sc-9959 to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 9
Date published: 2017-01-16
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Rated 4 out of 5 by from Antibody review This antibody worked with immunoblotting with mosue tissue lysate at 1:500 dilution.
Date published: 2018-06-14
Rated 4 out of 5 by from HDAC2 Good use for WB I test the antibody few months ago and it worked perfectly at 1/1000 overnight in milk.
Date published: 2017-12-05
Rated 5 out of 5 by from Excellent anti-HDAC2 (C-8) antibody This antibody worked well. We used it in western blot assay at 1:500. The band is single and clear.Very good!
Date published: 2017-10-30
Rated 5 out of 5 by from Result I bought this antibody a few months ago, it really fits the WB(1/800) and I see a signal are strong.
Date published: 2017-10-24
Rated 4 out of 5 by from Works very nice in Western I tested antibody by Western blotting and it seems to work very well.
Date published: 2017-09-27
Rated 5 out of 5 by from good quality This antibody worked well and generates a clear signal at the predicted position on the western blot (1:600 dilution, 50ug of rat heart extract).
Date published: 2017-09-21
Rated 4 out of 5 by from a relative good antibody for WB This antibody generates a clear signal at the predicted position on the western blot (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-01
Rated 5 out of 5 by from very good antibody for western blot. It works very well for western blot. Got a clear band.
Date published: 2017-08-14
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