HSPA5/BiP/GRP78 Antibody (E-4): sc-166490

GRP 78 Antibody (E-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects GRP 78 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GRP 78, also known as Binding Immunoglobulin Protein (BiP), is a crucial member of the heat shock protein 70 (HSP70) family, primarily localized in the endoplasmic reticulum (ER) of cells. GRP 78′s presence in the ER is vital as a molecular chaperone, ensuring correct folding and assembly of nascent proteins while maintaining protein homeostasis. This function is essential for preventing misfolded protein accumulation, which can lead to ER stress and activate the unfolded protein response (UPR), a critical cellular stress response associated with various diseases, including cancer and neurodegenerative disorders. GRP 78 interacts with several specific proteins, including ATF6, IRE1, and PERK, which are key players in the UPR pathway. Studying these interactions helps researchers better understand cellular stress response mechanisms and their implications in disease, making anti-GRP 78 antibody (E-4) an invaluable tool for advancing research in these important areas of biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HSPA5/BiP/GRP78 Antibody (E-4) References:

1. The role of Grp 78 in alpha 2-macroglobulin-induced signal transduction. Evidence from RNA interference that the low density lipoprotein receptor-related protein is associated with, but not necessary for, GRP 78-mediated signal transduction.  |  Misra, UK., et al. 2002. J Biol Chem. 277: 42082-7. PMID: 12194978
2. Identification of GRP 78 (BiP) as a liver cell expressed receptor element for dengue virus serotype 2.  |  Jindadamrongwech, S., et al. 2004. Arch Virol. 149: 915-27. PMID: 15098107
3. Endoplasmic reticulum chaperone protein GRP-78 mediates endocytosis of dentin matrix protein 1.  |  Ravindran, S., et al. 2008. J Biol Chem. 283: 29658-70. PMID: 18757373
4. GRP-78 secreted by tumor cells blocks the antiangiogenic activity of bortezomib.  |  Kern, J., et al. 2009. Blood. 114: 3960-7. PMID: 19713465
5. Stress chaperone GRP-78 functions in mineralized matrix formation.  |  Ravindran, S., et al. 2011. J Biol Chem. 286: 8729-39. PMID: 21239500
6. Expression and distribution of grp-78/bip in mineralizing tissues and mesenchymal cells.  |  Ravindran, S., et al. 2012. Histochem Cell Biol. 138: 113-25. PMID: 22527697
7. Turmeric and vitamin C mitigate testicular atrophy induced by lead diacetate via regulation of GRP-78/17β-HSD pathways in rat's model.  |  Fadda, LM., et al. 2021. Andrologia. 53: e14120. PMID: 34028854
8. The Clinicopathological Significance of BiP/GRP-78 in Breast Cancer: A Meta-Analysis of Public Datasets and Immunohistochemical Detection.  |  Direito, I., et al. 2022. Curr Oncol. 29: 9066-9087. PMID: 36547124
9. Corilagin enhances the anti-tumor activity of 5-FU by downregulating the expression of GRP 78.  |  Li, S., et al. 2023. Sci Rep. 13: 22661. PMID: 38114593
10. Cell surface localization of the 78 kD glucose regulated protein (GRP 78) induced by thapsigargin.  |  Delpino, A., et al. 1998. Mol Membr Biol. 15: 21-6. PMID: 9595551

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSPA5/BiP/GRP78 Antibody (E-4) has 71 citations in a variety of scientific publications.

Primary antibodies like Anti-HSPA5/BiP/GRP78 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSPA5/BiP/GRP78 Antibody (E-4) Specifications

Alternate Product Name: GRP78

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 19-50

Epitope Target: GRP 78

Epitope Species: human

Product Reactivity/Detection: GRP 78

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 71 citations in various scientific publications

Available Conjugates/Forms for HSPA5/BiP/GRP78 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HSPA5/BiP/GRP78 antibody is HSPA5/BiP/GRP78 Antibody (76-E6): sc-13539.