HSPA5/BiP/GRP78 Antibody (76-E6): sc-13539

GRP 78 Antibody (76-E6) is a rat monoclonal IgG antibody raised against full-length GRP 78 of mouse origin, reactive with mouse, rat, and human proteins, and is suitable for applications such as western blotting (WB) and immunoprecipitation (IP). GRP 78, also known as BiP or HSPA5, is a member of the HSP70 family of heat shock proteins and functions as a molecular chaperone within the endoplasmic reticulum (ER). GRP 78 plays a crucial role in the proper folding and assembly of nascent polypeptide chains, preventing misfolded proteins from aggregating and assisting in their translocation across the ER membrane. This function is essential for maintaining cellular homeostasis and protein quality control, especially under conditions of ER stress caused by factors like hypoxia, nutrient deprivation, or viral infections. GRP 78 is also a key regulator of the unfolded protein response (UPR), a cellular stress response related to the ER that helps cells adapt to adverse conditions by enhancing their protein-folding capacity. GRP 78′s function is important because dysregulation of GRP 78 expression has been implicated in various diseases, including cancer, neurodegeneration, and diabetes. Overexpression of GRP 78 is often observed in tumor cells, where GRP 78 contributes to tumor survival, proliferation, and resistance to chemotherapy by aiding in the management of increased protein synthesis and folding demands. Studying GRP 78 is vital for understanding mechanisms of protein homeostasis, stress responses, and developing therapeutic strategies for diseases associated with ER stress and protein misfolding.

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HSPA5/BiP/GRP78 Antibody (76-E6) References:

1. Prevention of protein denaturation under heat stress by the chaperonin Hsp60.  |  Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
2. Effects of low culture temperature on the induction of hsp70 mRNA and the accumulation of hsp70 and hsp105 in mouse FM3A cells.  |  Hatayama, T., et al. 1992. J Biochem. 111: 484-90. PMID: 1618738
3. Cloning of rat grp75, an hsp70-family member, and its expression in normal and ischemic brain.  |  Massa, SM., et al. 1995. J Neurosci Res. 40: 807-19. PMID: 7629893
4. Protein-mediated protein maturation in eukaryotes.  |  Haas, IG. 1995. FEBS Lett. 369: 72-5. PMID: 7641888
5. Can Hsp70 proteins act as force-generating motors?  |  Glick, BS. 1995. Cell. 80: 11-4. PMID: 7813006
6. Cloning and subcellular localization of human mitochondrial hsp70.  |  Bhattacharyya, T., et al. 1995. J Biol Chem. 270: 1705-10. PMID: 7829505
7. Autophosphorylation of grp94 (endoplasmin).  |  Csermely, P., et al. 1995. J Biol Chem. 270: 6381-8. PMID: 7890776

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSPA5/BiP/GRP78 Antibody (76-E6) has 104 citations in a variety of scientific publications.

Primary antibodies like Anti-HSPA5/BiP/GRP78 Antibody (76-E6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSPA5/BiP/GRP78 Antibody (76-E6) Specifications

Alternate Product Name: GRP78

Product Clone Name: 76-E6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: GRP 78

Epitope Species: mouse

Product Reactivity/Detection: GRP 78

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 104 citations in various scientific publications

Available Conjugates/Forms for HSPA5/BiP/GRP78 Antibody (76-E6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HSPA5/BiP/GRP78 antibody is HSPA5/BiP/GRP78 Antibody (E-4): sc-166490.