GFP Antibody (F56-6A1): sc-53882

GFP Antibody (F56-6A1) is a mouse monoclonal IgG2b kappa light chain antibody, designed against the full-length green fluorescent protein (GFP) from Aequorea victoria. GFP, a luminescent protein, is integral to the bioluminescence of this jellyfish species and is widely used in biological research as a fluorescent reporter. Known for its ability to emit green light when exposed to blue or ultraviolet light, GFP is expressed in various cell types and organisms, producing fluorescence without the need for additional substrates or cofactors. GFP localizes primarily in the cytoplasm when expressed, making GFP an ideal marker for monitoring protein localization, dynamics, and interactions within live cells. Enhanced variants like EGFP exhibit improved fluorescence intensity and modified excitation/emission properties, expanding GFP′s utility in fluorescence microscopy, flow cytometry, and other imaging applications. GFP′s role as a fusion protein tag allows researchers to track the expression and behavior of target proteins in real time, facilitating insights into cellular mechanisms. Anti-GFP antibody (F56-6A1) is validated for applications including western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA), providing researchers with a robust tool for GFP detection and analysis.

The preferred GFP antibody is GFP Antibody (B-2): sc-9996.