GFP Antibody (B-2) recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA

GFP Antibody (B-2): sc-9996

GFP Antibody (B-2) is rated 4.8 out of 5 by 141.
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Datasheets

    • Anti-GFP Antibody (B-2) is a mouse monoclonal IgG2a (kappa light chain) GFP antibody provided at 200 µg/ml
    • raised against amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin
    • GFP Antibody (B-2) is recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA
    • Anti-GFP Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GFP (B-2): sc-9996.

What is the molecular weight that will show up on a western blot?

Asked by: jordanhollemb
Thank you for your question. The observed molecular weight of GFP is 27 kDa.
Answered by: Technical Support Europe
Date published: 2020-10-08

How many microliters does the vial have?

Asked by: César
Thank you for your question. GFP Antibody (B-2): sc-9996 is supplied as 200 µg antibody in 1 ml PBS with 0.1% gelatin and <0.1% sodium azide.
Answered by: Tech Support Europe
Date published: 2020-08-10

Hallo. kann der Antikörper sc-9996 auch an eYFP binden? Wenn ja, können Sie mir ein Referenz nennen? Vielen dank.

Asked by: jpvp
Vielen Dank für Ihre Anfrage. Die Proteinsequenzen von eYFP und Aequorea victoria GFP sind einander zwar sehr ähnlich, aber nicht zu 100 % identisch. Da bisher kein Epitopmapping für diesen Antikörper durchgeführt wurde, können wir nicht mit absoluter Sicherheit sagen, ob der Antikörper auch eYFP erkennen würde. Kontaktieren Sie gerne unseren technischen Service um ein Sample dieses Antikörpers zu arrangieren. Thank you for your question. The sequence against which GFP Antibody (B-2): sc-9996 has been raised is not 100 % identical with the sequence of eYFP. Since no epitope mapping has been performed for this antibody, we cannot say with absolute certainty whether it would recognize eYGFP. Please contact our technical service directly for more information or to arrange for a sample of this antibody to see if it is suitable for your application.
Answered by: Tech Support Europe
Date published: 2020-04-02

Does Sc9996 antibody need a secondary probing as it is HRP labelled...

Asked by: Sajad
Thank you for your question. If your antibody is HRP conjugated (the vial states sc-9996 HRP), then no, you do not need to use a secondary antibody.
Answered by: Tech Service
Date published: 2020-02-26

What is the suitable dilution for Sc 9996 antibody

Asked by: Sajad
Thank you for your question. The dilution varies depending on the application you wish to use the antibody for, but for Western blot, the recommended starting dilution is 1:100 and for IF/IHC, we recommend a starting dilution of 1:50. Please see the recommended protocols for further information and additional details: https://www.scbt.com/resources/protocols
Answered by: Tech Service
Date published: 2020-02-26

May we receive a sample of the sc-9996 (GFP Antibody (B-2): sc-9996)?

Asked by: Catj
Thank you for your question. Please contact Technical Service for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-09-24

Hola, Me gustaría saber qué significa la letra y el número que acompaña a vuestros anticuerpos (por ejemplo, anti-GFP B-2). Gracias

Asked by: Anonymous
Muchas gracias por su pregunta. La letra y el numero que acompañan nuestros anticuerpos representan el clon del hibridoma que se empleó para producir el anticuerpo (por ejemplo el anti-GFP). Por cualquier duda o consulta nuestro servicio tecnico está siempre a su disposición.
Answered by: Tech Support Europe
Date published: 2019-06-19

Does this antibody detect AcGFP1?

Asked by: user_na
Thank you for your question. Please contact our Technical Service Department for help with performing sequence analysis to predict if this antibody can detect your GFP protein of interest.
Answered by: Technical Support
Date published: 2019-10-24
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Rated 5 out of 5 by from Great for IF We performed IF staining of the epithelial cells that overexpress GFP
Date published: 2020-09-17
Rated 5 out of 5 by from Great 293T細胞にGFP融合タンパク質を発現させてWestern blotを行いました。綺麗に検出することができました。
Date published: 2020-08-24
Rated 5 out of 5 by from Good antibody You can get great western blot images by using this antibody.
Date published: 2020-08-13
Rated 2 out of 5 by from 検出はできましたが、感度が低めでした GFPを一過的に発現させた細胞のライセートを使ってWBしました。 この抗体で特異的なバンドが検出できましたが、感度が低めな印象でした。
Date published: 2020-08-04
Rated 4 out of 5 by from Good for WB We obtained good results of this antibody in western blot (1:500) in mouse intestine samples.
Date published: 2020-07-29
Rated 5 out of 5 by from This is a good antibody with western blotting This antibody very works with western blotting with SAS cells lysate.
Date published: 2020-06-12
Rated 5 out of 5 by from great Works very good on western for EGFP protein which was extracted from plant. work great with sc-516102 (m-IgGκ BP-HRP)
Date published: 2020-04-09
Rated 4 out of 5 by from Good Sample of antibody against gfp works very good on ecfp protein. However, antibody do not interact with eyfp.
Date published: 2020-01-12
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