Geminin Antibody (H-3): sc-374187

Geminin Antibody (H-3) is a mouse monoclonal IgG2b antibody that detects geminin in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Geminin is a crucial nuclear protein that plays a vital role in regulating DNA replication initiation during the cell cycle, ensuring DNA replicates only once per cycle to maintain genomic stability. Anti-geminin antibody (H-3) achieves this by inhibiting the assembly of minichromosome maintenance (MCM) proteins into the prereplication complex, effectively preventing premature DNA replication. Geminin expression levels are tightly regulated, peaking during the S, G2, and M phases of the cell cycle, while remaining lowest during the G1 phase. This fluctuation is essential, as geminin degradation during mitosis, mediated by the anaphase-promoting complex (APC), allows for the re-establishment of the replication machinery in the subsequent cell cycle. Precise control of geminin levels is critical for cellular function, as dysregulation can lead to genomic instability and contribute to oncogenesis. Anti-geminin antibody (H-3) serves as an invaluable tool for researchers studying cell cycle regulation and DNA replication mechanisms.

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Geminin Antibody (H-3) References:

1. Geminin as a molecular target for the development of new anticancer drugs.  |  Yoshida, K. 2006. Mini Rev Med Chem. 6: 461-2. PMID: 16613582
2. T cell proliferation and homeostasis: an emerging role for the cell cycle inhibitor geminin.  |  Karamitros, D., et al. 2011. Crit Rev Immunol. 31: 209-31. PMID: 21740351
3. Reduced Geminin levels promote cellular senescence.  |  Iliou, MS., et al. 2013. Mech Ageing Dev. 134: 10-23. PMID: 23142824
4. The potential utility of geminin as a predictive biomarker in breast cancer.  |  Sundara Rajan, S., et al. 2014. Breast Cancer Res Treat. 143: 91-8. PMID: 24292956
5. The dual roles of geminin during trophoblast proliferation and differentiation.  |  de Renty, C., et al. 2014. Dev Biol. 387: 49-63. PMID: 24412371
6. Geminin interference facilitates vascular smooth muscle cell proliferation by upregulation of CDK-1.  |  Zhang, Y., et al. 2014. Cardiovasc Drugs Ther. 28: 407-14. PMID: 25189787
7. Geminin deletion in pre-meiotic DNA replication stage causes spermatogenesis defect and infertility.  |  Yuan, Y., et al. 2017. J Reprod Dev. 63: 481-488. PMID: 28690291
8. The molecular underpinning of geminin-overexpressing triple-negative breast cancer cells homing specifically to lungs.  |  Sami, E., et al. 2022. Cancer Gene Ther. 29: 304-325. PMID: 33723406
9. Geminin is essential for DNA re-replication in the silk gland cells of silkworms.  |  Liu, TH., et al. 2022. Exp Cell Res. 410: 112951. PMID: 34843715
10. Alterations in the spatiotemporal expression pattern of geminin during human epidermal morphogenesis.  |  Pasmatzi, E., et al. 2023. Acta Dermatovenerol Alp Pannonica Adriat. 32: 1-5. PMID: 36945759

The preferred Geminin antibody is Geminin Antibody (F-7): sc-74456.