GCH-I Antibody (G-8): sc-376483

GCH-I Antibody (G-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects GCH-I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GCH-I (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GTP cyclohydrolase I (GCH-I) plays a crucial role as the first and rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin (BH4), which is essential for the production of neurotransmitters such as dopamine and serotonin. GCH-I functions by enabling BH4 to serve as a cofactor for tyrosine hydroxylase and tryptophan hydroxylase, both critical for synthesizing dopamine and serotonin, respectively. These neurotransmitters are vital for mood regulation, and deficiencies in BH4 can lead to conditions such as depression. Mutations in GCH-I are linked to serious neurological disorders, including malignant hyperphenylalaninemia and dopa-responsive dystonia, highlighting GCH-I′s significance in both metabolic pathways and neurological health.

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GCH-I Antibody (G-8) References:

1. GTP cyclohydrolase I feedback regulatory protein-dependent and -independent inhibitors of GTP cyclohydrolase I.  |  Yoneyama, T., et al. 2001. Arch Biochem Biophys. 388: 67-73. PMID: 11361142
2. Role of human GTP cyclohydrolase I and its regulatory protein in tetrahydrobiopterin metabolism.  |  Gesierich, A., et al. 2003. Basic Res Cardiol. 98: 69-75. PMID: 12607127
3. GTP cyclohydrolase I prevents diabetic-impaired endothelial progenitor cells and wound healing by suppressing oxidative stress/thrombospondin-1.  |  Tie, L., et al. 2014. Am J Physiol Endocrinol Metab. 306: E1120-31. PMID: 24644242
4. Erythropoietin prevents endothelial dysfunction in GTP-cyclohydrolase I-deficient hph1 mice.  |  d'Uscio, LV., et al. 2014. J Cardiovasc Pharmacol. 64: 514-21. PMID: 25490417
5. Elevated GTP Cyclohydrolase I Pathway in Endothelial Progenitor Cells of Overweight Premenopausal Women.  |  Wu, S., et al. 2020. Cardiol Res Pract. 2020: 5914916. PMID: 32377428
6. A hybrid approach reveals the allosteric regulation of GTP cyclohydrolase I.  |  Ebenhoch, R., et al. 2020. Proc Natl Acad Sci U S A. 117: 31838-31849. PMID: 33229582
7. Inhibition of Brain GTP Cyclohydrolase I Attenuates 3-Nitropropionic Acid-Induced Striatal Toxicity: Involvement of Mas Receptor/PI3k/Akt/CREB/ BDNF Axis.  |  Mustafa, AM., et al. 2021. Front Pharmacol. 12: 740966. PMID: 35002694
8. Human GTP cyclohydrolase I: only one out of three cDNA isoforms gives rise to the active enzyme.  |  Gütlich, M., et al. 1994. Biochem J. 302 (Pt 1): 215-21. PMID: 8068008
9. Purification and cloning of the GTP cyclohydrolase I feedback regulatory protein, GFRP.  |  Milstien, S., et al. 1996. J Biol Chem. 271: 19743-51. PMID: 8702680
10. Mutations in the GTP cyclohydrolase I and 6-pyruvoyl-tetrahydropterin synthase genes.  |  Thöny, B. and Blau, N. 1997. Hum Mutat. 10: 11-20. PMID: 9222755

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GCH-I Antibody (G-8) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-GCH-I Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GCH-I Antibody (G-8) Specifications

Alternate Product Name: GCH-I

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-250

Epitope Target: GCH-I

Epitope Species: human

Product Reactivity/Detection: GCH-I isoforms GCH-1, GCH-2, GCH-3 and GCH-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for GCH-I Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred GCH-I antibody is GCH-I Antibody (C-4): sc-271482.