GCH-I Antibody (C-4): sc-271482

GCH-I Antibody (C-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects GCH-I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GCH-I antibody (C-4) is available as the non-conjugated form. GTP cyclohydrolase I (GCH-I) plays a crucial role in the biosynthesis of tetrahydrobiopterin (BH4), which is essential for the production of neurotransmitters such as dopamine and serotonin. BH4 serves as a cofactor for tyrosine hydroxylase and tryptophan hydroxylase, the rate-limiting enzymes in the synthesis of these neurotransmitters. Proper functioning of GCH-I is vital, as deficiencies in BH4 can lead to neurological disorders, including depression, due to impaired neurotransmitter synthesis. Additionally, mutations in the GCH-I gene are linked to serious conditions such as malignant hyperphenylalaninemia and dopa-responsive dystonia, highlighting the importance of GCH-I in maintaining neurological health. GCH-I (C-4) antibody is an invaluable tool for researchers studying the biochemical pathways involving this critical enzyme.

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GCH-I Antibody (C-4) References:

1. GTP cyclohydrolase I is coinduced in hepatocytes stimulated to produce nitric oxide.  |  Geller, DA., et al. 2000. Biochem Biophys Res Commun. 276: 633-41. PMID: 11027524
2. Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP.  |  Maita, N., et al. 2002. Proc Natl Acad Sci U S A. 99: 1212-7. PMID: 11818540
3. Folate synthesis in plants: the first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I.  |  Basset, G., et al. 2002. Proc Natl Acad Sci U S A. 99: 12489-94. PMID: 12221287
4. Reduction of GTP cyclohydrolase I feedback regulating protein expression by hydrogen peroxide in vascular endothelial cells.  |  Ishii, M., et al. 2005. J Pharmacol Sci. 97: 299-302. PMID: 15699573
5. High mutation rate in dopa-responsive dystonia: detection with comprehensive GCHI screening.  |  Hagenah, J., et al. 2005. Neurology. 64: 908-11. PMID: 15753436
6. Gene symbol: GTP cyclohydrolase I (GCH-I). Disease: dopa-responsive dystonia.  |  Hui xie, H., et al. 2005. Hum Genet. 116: 235. PMID: 15818812
7. Pulmonary hypertension in a GTP-cyclohydrolase 1-deficient mouse.  |  Nandi, M., et al. 2005. Circulation. 111: 2086-90. PMID: 15824199
8. Linkage and candidate gene analysis of 14q22-24 in bipolar disorder: support for GCHI as a novel susceptibility gene.  |  Kealey, C., et al. 2005. Am J Med Genet B Neuropsychiatr Genet. 136B: 75-80. PMID: 15909293
9. Characterization of transgenic mice with the expression of phenylalanine hydroxylase and GTP cyclohydrolase I in the skin.  |  Christensen, R., et al. 2005. Exp Dermatol. 14: 535-42. PMID: 15946242
10. Nitric oxide formation in acutely rejecting cardiac allografts correlates with GTP cyclohydrolase I activity.  |  Pieper, GM., et al. 2005. Biochem J. 391: 541-7. PMID: 16000090
11. Endothelium-Specific GTP Cyclohydrolase I Overexpression Restores Endothelial Function in Aged Mice.  |  Yan, L., et al. 2021. J Vasc Res. 1-5. PMID: 33556943
12. Early-onset autosomal dominant GTP-cyclohydrolase I deficiency: Diagnostic delay and residual motor signs.  |  Kim, W., et al. 2021. Brain Dev. 43: 759-767. PMID: 33875303

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GCH-I Antibody (C-4) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-GCH-I Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GCH-I Antibody (C-4) Specifications

Alternate Product Name: GCH-I

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 210-238

Epitope Target: GCH-I

Epitope Species: human

Product Reactivity/Detection: GCH-I isoform GCH-1 only

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for GCH-I Antibody (C-4): P: Blocking Peptide

The preferred GCH-I antibody is GCH-I Antibody (G-8): sc-376483.