GAP1-InsP₄ BP Antibody (E-3): sc-515684

GAP1-InsP4 BP Antibody (E-3) is a mouse monoclonal IgG1 antibody that detects GAP1-InsP4 BP in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GAP1-InsP4 BP, also known as Ras p21 protein activator (GTPase-activating protein) 3, is an 829-amino acid protein that plays a crucial role in cellular signaling by binding phospholipids in both calcium-dependent and -independent manners. GAP1-InsP4 BP is a member of the Ras GTPase-activating protein family, which consists of four distinct genes, including GAP1(m), GAP1-InsP4 BP, MRASAL, and KIAA0538. The structural composition of GAP1-InsP4 BP includes an N-terminal tandem C2 domain, a GAP-related domain, and a C-terminal pleckstrin homology (PH) domain, with the PH domains being particularly important for membrane targeting as these domains facilitate the binding of specific phospholipids. Following the stimulation of phosphatidylinositol 3,4,5-trisphosphate production, GAP1-InsP4 BP selectively binds inositol phosphates, which are then directed to the plasma membrane, underscoring GAP1-InsP4 BP′s vital role in regulating cellular responses to external signals. GAP1-InsP4 BP (E-3) antibody is an essential tool for researchers investigating the intricate mechanisms of cell signaling and membrane dynamics.

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GAP1-InsP₄ BP Antibody (E-3) References:

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7. Nrf2 Sequesters Keap1 Preventing Podosome Disassembly: A Quintessential Duet Moonlights in Endothelium.  |  Kloska, D., et al. 2019. Antioxid Redox Signal. 30: 1709-1730. PMID: 30198307
8. Angio-associated migratory cell protein (AAMP) interacts with cell division cycle 42 (CDC42) and enhances migration and invasion in human non-small cell lung cancer cells.  |  Yao, S., et al. 2021. Cancer Lett. 502: 1-8. PMID: 33279622
9. Laminin-511 Activates the Human Induced Pluripotent Stem Cell Survival via α6β1 Integrin-Fyn-RhoA-ROCK Signaling.  |  Nakashima, Y. and Tsukahara, M. 2022. Stem Cells Dev. 31: 706-719. PMID: 35726387
10. Isolation of InsP4 and InsP6 binding proteins from human platelets: InsP4 promotes Ca2+ efflux from inside-out plasma membrane vesicles containing 104 kDa GAP1IP4BP protein.  |  O'Rourke, F., et al. 1996. Biochem J. 315 (Pt 3): 1027-34. PMID: 8645140

The preferred GAP1-InsP₄ BP antibody is GAP1-InsP₄ BP Antibody (E-9): sc-398283.