GAP1-InsP₄ BP Antibody (E-9): sc-398283

GAP1-InsP4 BP Antibody (E-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects GAP1-InsP4 BP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GAP1-InsP4 BP antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GAP1-InsP4 BP, also known as Ras p21 protein activator 3, is a crucial 829-amino acid protein that plays a significant role in cellular signaling by binding phospholipids in both calcium-dependent and -independent manners. GAP1-InsP4 BP belongs to the Ras GTPase-activating protein family, which consists of four distinct genes, including GAP1(m), GAP1-InsP4 BP, MRASAL, and KIAA0538. GAP1-InsP4 BP includes an N-terminal tandem C2 domain, a GAP-related domain, and a C-terminal pleckstrin homology (PH) domain, with the PH domain being essential for membrane targeting through specific phospholipid binding. Following the stimulation of phosphatidylinositol 3,4,5-trisphosphate production, GAP1-InsP4 BP selectively binds inositol phosphates, facilitating their localization to the plasma membrane, which is vital for the regulation of various signaling pathways and cellular responses.

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GAP1-InsP₄ BP Antibody (E-9) References:

1. Molecular modelling and site-directed mutagenesis of the inositol 1,3,4,5-tetrakisphosphate-binding pleckstrin homology domain from the Ras GTPase-activating protein GAP1IP4BP.  |  Cozier, G., et al. 2000. Biochem J. 349: 333-42. PMID: 10861245
2. GAP1IP4BP contains a novel group I pleckstrin homology domain that directs constitutive plasma membrane association.  |  Cozier, GE., et al. 2000. J Biol Chem. 275: 28261-8. PMID: 10869341
3. Distinct phosphoinositide binding specificity of the GAP1 family proteins: characterization of the pleckstrin homology domains of MRASAL and KIAA0538.  |  Minagawa, T., et al. 2001. Biochem Biophys Res Commun. 288: 87-90. PMID: 11594756
4. Comparative microarray analysis of gene expression during activation of human peripheral blood T cells and leukemic Jurkat T cells.  |  Lin, Z., et al. 2003. Lab Invest. 83: 765-76. PMID: 12808112
5. Pituitary tumor-transforming 1 increases cell motility and promotes lymph node metastasis in esophageal squamous cell carcinoma.  |  Ito, T., et al. 2008. Cancer Res. 68: 3214-24. PMID: 18451147
6. Proteomics analysis of malignant and benign prostate tissue by 2D DIGE/MS reveals new insights into proteins involved in prostate cancer.  |  Davalieva, K., et al. 2015. Prostate. 75: 1586-600. PMID: 26074449
7. ARHGAP1 overexpression inhibits proliferation, migration and invasion of C-33A and SiHa cell lines.  |  Li, JP., et al. 2017. Onco Targets Ther. 10: 691-701. PMID: 28223826
8. Exosome miR-134-5p restrains breast cancer progression via regulating PI3K/AKT pathway by targeting ARHGAP1.  |  Yang, C., et al. 2021. J Obstet Gynaecol Res. 47: 4037-4048. PMID: 34378285
9. circRNA RPPH1 Facilitates the Aggravation of Breast Cancer Development by Regulating miR-542-3p/ARHGAP1 Pathway.  |  Qi, L., et al. 2022. Cancer Biother Radiopharm. 37: 708-719. PMID: 34402683
10. ARHGAP1 Transported with Influenza Viral Genome Ensures Integrity of Viral Particle Surface through Efficient Budozone Formation.  |  Kuroki, T., et al. 2022. mBio. 13: e0072122. PMID: 35475647
11. Whole transcriptome expression array analysis of human colon fibroblasts culture treated with Helichrysum italicum supports its use in traditional medicine.  |  Kramberger, K., et al. 2022. J Ethnopharmacol. 296: 115505. PMID: 35764197
12. The Role of ARHGAP1 in Rho GTPase Inactivation during Metastasizing of Breast Cancer Cell Line MCF-7 after Treatment with Doxorubicin.  |  Géci, I., et al. 2023. Int J Mol Sci. 24: PMID: 37511111

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAP1-InsP₄ BP Antibody (E-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GAP1-InsP₄ BP Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAP1-InsP₄ BP Antibody (E-9) Specifications

Alternate Product Name: GAP1-InsP4 BP

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 103-136

Epitope Target: GAP1-InsP₄ BP

Epitope Species: human

Product Reactivity/Detection: GAP1-InsP₄ BP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GAP1-InsP₄ BP Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin