γ-GCSc Antibody (F-9): sc-166356

γ-GCSc Antibody (F-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects γ-GCSc in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). γ-GCSc (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The GCLC gene, which encodes the 636 amino acid protein γ-GCSc (also known as the γ-glutamylcysteine synthetase heavy subunit), consists of 16 exons and plays a crucial role in the synthesis of glutathione, a vital antioxidant that protects cells from oxidative stress. γ-GCSc is predominantly expressed in hemocytes, brain, liver, and kidney, where γ-GCSc forms a heterodimer with the regulatory subunit γ-GCSm (γ-glutamylcysteine synthetase light subunit) to create the γ-GCS enzyme complex. This complex is the first enzyme in the glutathione biosynthesis pathway and is responsible for the rate-limiting step in the production of this essential tripeptide. The regulation of γ-GCSc is influenced by various factors, including oxidants and heavy metals, which can enhance transcription, thereby increasing glutathione levels and potentially providing protective effects against chemically induced carcinogenesis. Notably, a His370Leu amino acid substitution in γ-GCSc has been linked to reduced enzymatic activity, resulting in hemolytic anemia and diminished glutathione levels in red blood cells, highlighting the importance of this protein in maintaining cellular health and function.

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γ-GCSc Antibody (F-9) References:

1. Nrf2 and c-Jun regulation of antioxidant response element (ARE)-mediated expression and induction of gamma-glutamylcysteine synthetase heavy subunit gene.  |  Jeyapaul, J. and Jaiswal, AK. 2000. Biochem Pharmacol. 59: 1433-9. PMID: 10751553
2. Activation of the Nrf2 pathway, but decreased gamma-glutamylcysteine synthetase heavy subunit chain levels and caspase-3-dependent apoptosis during exposure of primary mouse hepatocytes to diphenylarsinic acid.  |  Sumi, D., et al. 2007. Toxicol Appl Pharmacol. 223: 218-24. PMID: 17628625
3. Glucagon-like peptide-1 (GLP-1) protects against methylglyoxal-induced PC12 cell apoptosis through the PI3K/Akt/mTOR/GCLc/redox signaling pathway.  |  Kimura, R., et al. 2009. Neuroscience. 162: 1212-9. PMID: 19463904
4. Down-regulation of GCLC is involved in microcystin-LR-induced malignant transformation of human liver cells.  |  Jia, X., et al. 2019. Toxicology. 421: 49-58. PMID: 30940545
5. Downregulation of LncRNA GCLC-1 Promotes Microcystin-LR-Induced Malignant Transformation of Human Liver Cells by Regulating GCLC Expression.  |  Huang, X., et al. 2023. Toxics. 11: PMID: 36851037
6. Endocrine pancreas-specific Gclc gene deletion causes a severe diabetes phenotype.  |  Davidson, EA., et al. 2023. bioRxiv.. PMID: 37398356
7. Identification of a putative antioxidant response element in the 5'-flanking region of the human gamma-glutamylcysteine synthetase heavy subunit gene.  |  Mulcahy, RT. and Gipp, JJ. 1995. Biochem Biophys Res Commun. 209: 227-33. PMID: 7726839
8. Transcriptional regulation of gamma-glutamylcysteine synthetase-heavy subunit by oxidants in human alveolar epithelial cells.  |  Rahman, I., et al. 1996. Biochem Biophys Res Commun. 229: 832-7. PMID: 8954980
9. Constitutive and beta-naphthoflavone-induced expression of the human gamma-glutamylcysteine synthetase heavy subunit gene is regulated by a distal antioxidant response element/TRE sequence.  |  Mulcahy, RT., et al. 1997. J Biol Chem. 272: 7445-54. PMID: 9054446
10. Identification of cis-acting DNA elements of the human gamma-glutamylcysteine synthetase heavy subunit gene.  |  Tomonari, A., et al. 1997. Biochem Biophys Res Commun. 232: 522-7. PMID: 9125214

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-γ-GCSc Antibody (F-9) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-γ-GCSc Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

γ-GCSc Antibody (F-9) Specifications

Alternate Product Name: GCLC (glutamate cysteine ligase catalytic)

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 338-637

Epitope Target: γ-GCSc

Epitope Species: human

Product Reactivity/Detection: γ-GCSc

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for γ-GCSc Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred γ-GCSc antibody is γ-GCSc Antibody (H-5): sc-390811.