gamma-GCSc Antibody (H-5) recommended for detection of γ-GCSc of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

γ-GCSc Antibody (H-5): sc-390811

γ-GCSc Antibody (H-5) is rated 5.0 out of 5 by 8.
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Datasheets

    • Anti-γ-GCSc Antibody (H-5) is a mouse monoclonal IgG2a (kappa light chain) γ-GCSc antibody provided at 200 µg/ml
    • raised against amino acids 338-637 of γ-GCSc of human origin
    • Anti-gamma-GCSc Antibody (H-5) is recommended for detection of γ-GCSc of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-gamma-GCSc Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of γ-GCSc (H-5): sc-390811.

I am using γ-GCSc (H-5): sc-390811 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good in WB WB on 10µg of total mouse heart protein extract. Gel 10%, single band detected at 1/1000 and 1/10000 for secondary Ab
Date published: 2017-10-21
Rated 5 out of 5 by from This antibody is pretty good. This antibody is pretty good in WB, which worked well in a concentration of 1:1000 in rat intestinal tissue whole protein. 10% resolving gel concentration was used.
Date published: 2017-09-05
Rated 5 out of 5 by from GCSC ANTIBODY Nice Western Blot data of -GCSc expression in TM4 cell lysates by duilution at 1:500.
Date published: 2017-08-31
Rated 5 out of 5 by from 可以 抗体在90kd左右有一条挺深的条带,这个抗体的效果还可以,背景干净,我所使用的是rat来源的样品。。
Date published: 2017-07-11
Rated 5 out of 5 by from a review for GCSC antibody a good antibody,we used it for WB in cancer cells,such as h1299,when dilution in 1:1000,exposure just need 1 min.the band is about 70-80 KD.
Date published: 2017-03-13
Rated 5 out of 5 by from Nice bands by antibody in western blot We used this antibody for expression detection with western blot. We got nice bands at the correct molecular weight
Date published: 2017-01-31
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-01-18
Rated 5 out of 5 by from Nice Western Blot data of Nice Western Blot data of -GCSc expression in Hep G2 and A549 whole cell lysates. -SCBT QC
Date published: 2013-11-28
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