GADD 34 Antibody (B-10): sc-373815

GADD 34 Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects GADD 34 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-GADD 34 antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GADD 34, also known as PPP1R15A, plays a crucial role in cellular response to stress, particularly in DNA damage context. GADD 34 functions as a regulatory protein that interacts with protein phosphatase 1 (PP1), facilitating dephosphorylation of eukaryotic translation initiation factor 2 alpha (eIF2α). This action is vital because eIF2α phosphorylation state determines protein synthesis during stress conditions; when eIF2α is phosphorylated, global protein synthesis reduces, allowing cells to conserve resources and focus on repairing damage. GADD 34′s ability to modulate this pathway underscores its importance in maintaining cellular homeostasis and preventing damaged DNA propagation, which can lead to tumorigenesis. GADD 34 also interacts with other proteins involved in stress responses, highlighting its role in cellular signaling networks.

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GADD 34 Antibody (B-10) References:

1. Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha.  |  Novoa, I., et al. 2001. J Cell Biol. 153: 1011-22. PMID: 11381086
2. Induction of GADD34 Regulates the Neurotoxicity of Amyloid β.  |  Xu, N., et al. 2015. Am J Alzheimers Dis Other Demen. 30: 313-9. PMID: 25204313
3. GADD34 suppresses lipopolysaccharide-induced sepsis and tissue injury through the regulation of macrophage activation.  |  Ito, S., et al. 2016. Cell Death Dis. 7: e2219. PMID: 27171261
4. GADD34 Ablation Exacerbates Retinal Degeneration in P23H RHO Mice.  |  Saltykova, IV., et al. 2022. Int J Mol Sci. 23: PMID: 36430227
5. G1 phase progression: cycling on cue.  |  Sherr, CJ. 1994. Cell. 79: 551-5. PMID: 7954821
6. Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age.  |  Hunter, T. and Pines, J. 1994. Cell. 79: 573-82. PMID: 7954824
7. Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen.  |  Smith, ML., et al. 1994. Science. 266: 1376-80. PMID: 7973727
8. Inducible growth arrest: new mechanistic insights.  |  Ron, D. 1994. Proc Natl Acad Sci U S A. 91: 1985-6. PMID: 8134335
9. The novel primary response gene MyD118 and the proto-oncogenes myb, myc, and bcl-2 modulate transforming growth factor beta 1-induced apoptosis of myeloid leukemia cells.  |  Selvakumaran, M., et al. 1994. Mol Cell Biol. 14: 2352-60. PMID: 8139540
10. The gadd and MyD genes define a novel set of mammalian genes encoding acidic proteins that synergistically suppress cell growth.  |  Zhan, Q., et al. 1994. Mol Cell Biol. 14: 2361-71. PMID: 8139541
11. Effect of UV-irradiation on cell cycle, viability and the expression of p53, gadd153 and gadd45 genes in normal and HPV-immortalized human oral keratinocytes.  |  Gujuluva, CN., et al. 1994. Oncogene. 9: 1819-27. PMID: 8208528
12. Subtraction hybridization identifies a transformation progression-associated gene PEG-3 with sequence homology to a growth arrest and DNA damage-inducible gene.  |  Su, ZZ., et al. 1997. Proc Natl Acad Sci U S A. 94: 9125-30. PMID: 9256446

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GADD 34 Antibody (B-10) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-GADD 34 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GADD 34 Antibody (B-10) Specifications

Alternate Product Name: GADD34

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 633-660

Epitope Target: GADD 34

Epitope Species: human

Product Reactivity/Detection: GADD 34

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for GADD 34 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin