DDIT3/GADD153/CHOP Antibody (9C8): sc-56107

GADD 153 Antibody (9C8) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect GADD 153 protein, also known as CHOP or DDIT3, across mouse, rat, and human species using western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP) techniques. GADD 153 monoclonal antibody (9C8) targets an epitope derived from the mouse origin of GADD 153 and is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, to suit diverse experimental needs. GADD 153 is a crucial C/EBP-related nuclear transcription factor localized within the cell nucleus, where GADD 153 plays a pivotal role in regulating gene expression in response to cellular stressors such as nutrient deprivation and metabolic disturbances. By residing in the nucleus, GADD 153 can effectively interact with other transcription factors, facilitating its function in halting cell cycle progression from the G1 to S phase. GADD 153 achieves this by forming dimers with other C/EBP proteins, thereby redirecting these complexes from classical C/EBP binding sites to unique nonclassical sites, acting as a negative modulator of C/EBP-like proteins in terminally differentiated cells. This regulatory mechanism is analogous to how Id proteins modulate the activity of MyoD and related proteins during muscle cell development. Understanding the nuclear localization of GADD 153 is essential, as this localization underscores GADD 153′s role in orchestrating cellular responses to stress at the transcriptional level, making anti-GADD 153 antibody (9C8) an invaluable tool for researchers investigating cell cycle regulation, stress response pathways, and related molecular mechanisms.

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DDIT3/GADD153/CHOP Antibody (9C8) References:

1. The FUS-DDIT3 Interactome in Myxoid Liposarcoma.  |  Yu, JSE., et al. 2019. Neoplasia. 21: 740-751. PMID: 31220736
2. DDIT3 modulates cancer stemness in gastric cancer by directly regulating CEBPβ.  |  Lin, H., et al. 2020. J Pharm Pharmacol. 72: 807-815. PMID: 32189359
3. DDIT3/CHOP promotes autophagy in chondrocytes via SIRT1-AKT pathway.  |  Yang, C., et al. 2021. Biochim Biophys Acta Mol Cell Res. 1868: 119074. PMID: 34087318
4. DDIT3 Directs a Dual Mechanism to Balance Glycolysis and Oxidative Phosphorylation during Glutamine Deprivation.  |  Li, M., et al. 2021. Adv Sci (Weinh). 8: e2003732. PMID: 34105294
5. DDIT3/CHOP mediates the inhibitory effect of ER stress on chondrocyte differentiation by AMPKα-SIRT1 pathway.  |  Yu, X., et al. 2022. Biochim Biophys Acta Mol Cell Res. 1869: 119265. PMID: 35381294
6. The transcription factor DDIT3 is a potential driver of dyserythropoiesis in myelodysplastic syndromes.  |  Berastegui, N., et al. 2022. Nat Commun. 13: 7619. PMID: 36494342
7. DDIT3 aggravates pulpitis by modulating M1 polarization through EGR1 in macrophages.  |  Wang, Y., et al. 2023. Int Immunopharmacol. 120: 110328. PMID: 37235961
8. DDIT3 deficiency accelerates bone remodeling during bone healing by enhancing osteoblast and osteoclast differentiation through ULK1-mediated autophagy.  |  Jia, M., et al. 2024. Bone. 182: 117058. PMID: 38408589
9. DDIT3/CHOP promotes LPS/ATP-induced pyroptosis in osteoblasts via mitophagy inhibition.  |  Dong, Z., et al. 2024. Biochim Biophys Acta Mol Cell Res. 1871: 119712. PMID: 38521466
10. DDIT3 aggravates TMJOA cartilage degradation via Nrf2/HO-1/NLRP3-mediated autophagy.  |  Yang, C., et al. 2024. Osteoarthritis Cartilage. 32: 921-937. PMID: 38719085

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDIT3/GADD153/CHOP Antibody (9C8) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-DDIT3/GADD153/CHOP Antibody (9C8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDIT3/GADD153/CHOP Antibody (9C8) Specifications

Alternate Product Name: GADD 153

Product Clone Name: 9C8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: GADD 153

Epitope Species: mouse

Product Reactivity/Detection: GADD 153

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for DDIT3/GADD153/CHOP Antibody (9C8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred DDIT3/GADD153/CHOP antibody is DDIT3/GADD153/CHOP Antibody (B-3): sc-7351.