Flg Antibody (M5G10) recommended for detection of Flg isoforms α and β of mouse, rat and human origin by WB, IP, IF and IHC(P)

Flg Antibody (M5G10): sc-57133

Flg Antibody (M5G10) is rated 4.5 out of 5 by 2.
  • y_2020, m_4, d_7, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_57133, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 149ms
  • REVIEWS, PRODUCT
5
1
4
1
3
0
2
0
1
0

Datasheets

    • Flg Antibody (M5G10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against the ectodomain of Flg of human origin
    • recommended for detection of Flg isoforms α and β of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Flg (M2F12): sc-57132 for Flg antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
Submit a review for this product and receive 15 CruzCredits

How much sample do you recommend loading on the gel for Western blot detection using Flg (M5G10): sc-57133?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
  • y_2020, m_4, d_7, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_57133, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 128ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Sharp bands on human VSMCs the sample antibody gives very sharp bands at ~90 kDa, a little bit smaller than that is predicted, and that showed in the datasheet
Date published: 2017-07-20
Rated 4 out of 5 by from Good specificity in WB in human Flg transfected Good specificity in WB in human Flg transfected 293T whole cell lysate. -SCBT QC
Date published: 2013-08-26
  • y_2020, m_4, d_7, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_57133, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.