Flg Antibody (M2F12) recommended for detection of Flg isoform α of mouse, rat and human origin by WB, IP, IF and IHC(P)

Flg Antibody (M2F12): sc-57132

Flg Antibody (M2F12) is rated 4.5 out of 5 by 11.
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Datasheets

    • Anti-Flg Antibody (M2F12) is a mouse monoclonal IgG2a (kappa light chain) Flg antibody provided at 200 µg/ml
    • raised against the ectodomain of Flg isoform α of human origin
    • Anti-Flg Antibody (M2F12) is recommended for detection of Flg isoform α of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Flg Antibody (M2F12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Flg (M2F12): sc-57132.
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Can this antibody detect FGFR1 isoform beta? I'm trying to blot for endogenous FGFR1 in PC3 cell line, and I'm not getting a signal so I wonder if PC3 cells are expressing only beta form and the antibody cannot detect or something.

Asked by: Juko
Thank you very much for your question. This antibody was raised against Flg isoform α (ectodomain) of human origin. Unfortunately, we have not tested this antibody on PC3 cells. Our labs suggest as positive controls e.g. PC-12, HeLa, SK-N-SH, Neuro-2A and U-87 MG whole cell lysates (we do provide all these in case you are interested). Please contact our Technical Service Department by phone, e-mail or live chat in case you feel the WB results could be improved by changing the protocol settings. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application and get a signal at the expected mw size in a positive sample material, we will send you a replacement antibody free of charge.
Answered by: Technical Support Europe
Date published: 2017-09-18

Can Flg (M2F12): sc-57132 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Flg (M2F12): sc-57132 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-09-15
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Rated 5 out of 5 by from work well in western blot at 1:200,ov90 cell Although it looked like just so so.But it actually worked very well, so it is good
Date published: 2017-09-20
Rated 5 out of 5 by from Immunofluorescence I used it (Flg (M2F12)) for immunofluorescence at LX-2 cell (dilution 1:200), and appeared blight image.
Date published: 2017-07-05
Rated 5 out of 5 by from Great antibody The antibody is excellent in Western Blotting and IHC
Date published: 2017-03-22
Rated 5 out of 5 by from Strong antibody for IHC and WB Nice specific staining on paraffin sections, very clean and strong results. It's also a great choice for Western blot! Smart choice!
Date published: 2017-02-01
Rated 5 out of 5 by from Pulito e molto specifico Ho ricevuto 10 µg di anticorpo in prova e sono riuscito a vedere un segnale di FGFR1 finalmente pulito, chiaro ed inconfutabile. Mi ha risolto un problema che andava avanti da mesi.
Date published: 2017-01-02
Rated 4 out of 5 by from Positive band detected in WB in K Positive band detected in WB in K-562 whole cell lysate. -SCBT Publication Review
Date published: 2015-05-10
Rated 4 out of 5 by from Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-06
Rated 4 out of 5 by from Good band detected in WB in HeLa whole cell lysate Good band detected in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-22
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