FIH-1 Antibody (F-11): sc-365128
- FIH-1 Antibody (F-11) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 15-243 mapping within an internal region of FIH-1 of human origin
- recommended for detection of FIH-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for FIH-1 Antibody (F-11) for WB applications. These reagents are now offered in bundles with FIH-1 Antibody (F-11) (see ordering information below).
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FIH-1 Antibody (F-11) is a mouse monoclonal IgG2b antibody that detects Factor inhibiting HIF-1 (FIH-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-FIH-1 antibody (F-11) plays a crucial role in studying the regulation of hypoxia-inducible factor 1 alpha (HIF-1α) by functioning as an asparaginyl hydroxylase, specifically catalyzing the hydroxylation of asparagine residue 803 within the carboxy-terminal transactivation domain of HIF-1α. This post-translational modification is significant as FIH-1 (F-11) antibody helps researchers understand how FIH-1 inhibits the interaction of HIF-1α with essential co-activators, thereby modulating the transcriptional activity of genes involved in the cellular response to hypoxia. Additionally, FIH-1 monoclonal antibody (F-11) enables research into FIH-1′s interaction with the von Hippel-Lindau (VHL) tumor suppressor protein, further contributing to the regulation of HIF-1α activity. Studies using FIH-1 (F-11) antibody have revealed FIH-1′s localization in the cytoplasm of transiently transfected human osteosarcoma cells, underscoring its functional importance in cellular signaling pathways. The structural characteristics of FIH-1, including its jellyroll-like β-barrel and ferrous-binding triad residues, are vital for its enzymatic activity and stability, making FIH-1 (F-11) monoclonal antibody an essential tool for researchers studying hypoxia-related processes and their implications in various diseases.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FIH-1 Antibody (F-11) | sc-365128 | 200 µg/ml | $322.00 | |||
FIH-1 Antibody (F-11): m-IgGκ BP-HRP Bundle | sc-535155 | 200 µg Ab; 40 µg BP | $361.00 | |||
FIH-1 Antibody (F-11): m-IgG2b BP-HRP Bundle | sc-549348 | 200 µg Ab; 10 µg BP | $361.00 |