FIH-1 Antibody (A-5): sc-271780

FIH-1 Antibody (A-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects FIH-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). FIH-1 (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Factor Inhibiting HIF-1 (FIH-1) plays a crucial role in cellular response to hypoxia by regulating hypoxia-inducible factor 1 alpha (HIF-1α) activity. FIH-1 functions as an asparaginyl hydroxylase, catalyzing the hydroxylation of asparagine residue 803 within the carboxy-terminal transactivation domain of HIF-1α, which prevents interaction with co-activators and thus inhibits transcriptional activity. This regulatory mechanism is vital for maintaining cellular homeostasis under varying oxygen levels, as dysregulation of HIF-1α can lead to pathological conditions such as cancer and ischemia. FIH-1 also interacts with von Hippel-Lindau (VHL) tumor suppressor protein, further contributing to HIF-1α activity repression. In transiently transfected human osteosarcoma cells, FIH-1 is localized in the cytoplasm, and FIH-1 structure features a jellyroll-like β-barrel containing ferrous-binding triad residues, essential for enzymatic function. Human FIH-1 gene is located on chromosome 10q24, highlighting significance in genetic regulation and potential implications in disease.

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FIH-1 Antibody (A-5) References:

1. FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity.  |  Mahon, PC., et al. 2001. Genes Dev. 15: 2675-86. PMID: 11641274
2. FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor.  |  Lando, D., et al. 2002. Genes Dev. 16: 1466-71. PMID: 12080085
3. Hypoxia-inducible factor asparaginyl hydroxylase (FIH-1) catalyses hydroxylation at the beta-carbon of asparagine-803.  |  McNeill, LA., et al. 2002. Biochem J. 367: 571-5. PMID: 12215170
4. Structure of factor-inhibiting hypoxia-inducible factor 1: An asparaginyl hydroxylase involved in the hypoxic response pathway.  |  Dann, CE., et al. 2002. Proc Natl Acad Sci U S A. 99: 15351-6. PMID: 12432100
5. Intracellular localisation of human HIF-1 alpha hydroxylases: implications for oxygen sensing.  |  Metzen, E., et al. 2003. J Cell Sci. 116: 1319-26. PMID: 12615973

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FIH-1 Antibody (A-5) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-FIH-1 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FIH-1 Antibody (A-5) Specifications

Alternate Product Name: Factor Inhibiting HIF-1

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 15-243

Epitope Target: FIH-1

Epitope Species: human

Product Reactivity/Detection: FIH-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for FIH-1 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin