Fhit Antibody (G-4): sc-390481

Fhit Antibody (G-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Fhit protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Fhit (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fhit, a member of the histidine triad (HIT) superfamily, plays a crucial role as a dinucleoside 5′,5′′′-P1,P3-triphosphate hydrolase, which regulates nucleotide metabolism and maintains cellular homeostasis. Fhit function is particularly important in tumor suppression, as alterations in FHIT gene structure and expression have been implicated in various cancers, including esophageal, head and neck, lung, gastric, breast, and cervical carcinomas. FHIT gene is located at the FRA3B common fragile site, making Fhit highly susceptible to damage from carcinogens, and the unique pattern of mutational inactivation observed in FHIT gene distinguishes Fhit from other known tumor suppressors. Studies have shown that loss of FHIT exons correlates with smoking duration and asbestos exposure, highlighting Fhit′s potential as a biomarker for cancer risk.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Fhit Antibody (G-4) References:

1. Fhit Nuclear Import Following EGF Stimulation Sustains Proliferation of Breast Cancer Cells.  |  Bianchi, F., et al. 2015. J Cell Physiol. 230: 2661-70. PMID: 25711523
2. Fhit loss-associated initiation and progression of neoplasia in vitro.  |  Karras, JR., et al. 2016. Cancer Sci. 107: 1590-1598. PMID: 27513973
3. Identification of Fhit as a post-transcriptional effector of Thymidine Kinase 1 expression.  |  Kiss, DL., et al. 2017. Biochim Biophys Acta Gene Regul Mech. 1860: 374-382. PMID: 28093273
4. FHIT, a Novel Modifier Gene in Pulmonary Arterial Hypertension.  |  Dannewitz Prosseda, S., et al. 2019. Am J Respir Crit Care Med. 199: 83-98. PMID: 30107138
5. System analysis of FHIT in LUAD and LUSC: The expression, prognosis, gene regulation network, and regulation targets.  |  Situ, Y., et al. 2022. Int J Biol Markers. 37: 158-169. PMID: 35254116
6. Fhit, a putative tumor suppressor in humans, is a dinucleoside 5',5''-P1,P3-triphosphate hydrolase.  |  Barnes, LD., et al. 1996. Biochemistry. 35: 11529-35. PMID: 8794732
7. Frequent abnormalities of FHIT, a candidate tumor suppressor gene, in head and neck cancer cell lines.  |  Mao, L., et al. 1996. Cancer Res. 56: 5128-31. PMID: 8912845
8. FHIT gene and the FRA3B region are not involved in the genetics of renal cell carcinomas.  |  Bugert, P., et al. 1997. Genes Chromosomes Cancer. 20: 9-15. PMID: 9290948
9. Frequent deletions of FHIT and FRA3B in Barrett's metaplasia and esophageal adenocarcinomas.  |  Michael, D., et al. 1997. Oncogene. 15: 1653-9. PMID: 9349498
10. Replacement of Fhit in cancer cells suppresses tumorigenicity.  |  Siprashvili, Z., et al. 1997. Proc Natl Acad Sci U S A. 94: 13771-6. PMID: 9391102
11. An FHIT tumor suppressor gene?  |  Le Beau, MM., et al. 1998. Genes Chromosomes Cancer. 21: 281-9. PMID: 9559339
12. Chromosome 3p14 alterations in lung cancer: evidence that FHIT exon deletion is a target of tobacco carcinogens and asbestos.  |  Nelson, HH., et al. 1998. Cancer Res. 58: 1804-7. PMID: 9581816

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fhit Antibody (G-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Fhit Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fhit Antibody (G-4) Specifications

Alternate Product Name: Fhit

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-107

Epitope Target: Fhit

Epitope Species: human

Product Reactivity/Detection: Fhit

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Fhit Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin