ERK 1 Antibody (E-6): sc-271270

ERK 1 Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody specific for an epitope mapping between amino acids 291-335 within subdomain XI of ERK 1 of rat origin, exhibiting strong species reactivity with mouse, rat, and human proteins by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA); anti-ERK1 antibody (E-6) is also reactive with additional species including equine, canine, bovine, and porcine. ERK1, also known as Mitogen-activated protein kinase 3 (MAPK3), is a crucial component of the MAPK/ERK signaling pathway, which transmits signals from the cell surface to the nucleus in response to various extracellular stimuli such as growth factors, hormones, and cytokines. ERK1 interacts with a variety of proteins that regulate activation and function. Upstream, ERK1 is activated by dual phosphorylation by MAP kinase kinases MEK1 and MEK2, which themselves are activated by Raf kinases like Raf-1. ERK1 interacts with scaffold proteins such as KSR1 and MP1, which facilitate the assembly of the MAPK signaling module, ensuring specificity and efficiency of signal transduction. Once activated, ERK1 translocates to the nucleus where ERK1 interacts with and phosphorylates numerous transcription factors, including ELK1, c-Fos, and c-Jun, leading to the regulation of genes involved in cell proliferation, differentiation, and survival. ERK1 also interacts with downstream kinases such as RSK (ribosomal S6 kinase), which phosphorylate additional substrates involved in protein synthesis and cell growth. ERK1 interactions with these proteins are essential for the propagation and modulation of cellular responses to external stimuli, and dysregulation of ERK1 signaling is implicated in various diseases, including cancer and neurodegenerative disorders. ERK1 monoclonal antibody (E-6) is an invaluable tool for researchers studying ERK1 interactions and the role of the MAPK/ERK pathway in health and disease.

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ERK 1 Antibody (E-6) References:

1. Signaling angiogenesis via p42/p44 MAP kinase cascade.  |  Pagès, G., et al. 2000. Ann N Y Acad Sci. 902: 187-200. PMID: 10865838
2. Regulation of mitogen-activated protein kinase signaling networks by G protein-coupled receptors.  |  Gutkind, JS. 2000. Sci STKE. 2000: re1. PMID: 11752597
3. Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling.  |  Pouysségur, J., et al. 2002. Biochem Pharmacol. 64: 755-63. PMID: 12213567
4. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
5. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
6. Targeting extracellular signal-regulated kinase (ERK) signaling has therapeutic implications for inflammatory osteolysis.  |  Seo, SW., et al. 2010. Bone. 46: 695-702. PMID: 19895919
7. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
8. ERK phosphorylation and nuclear accumulation: insights from single-cell imaging.  |  Caunt, CJ. and McArdle, CA. 2012. Biochem Soc Trans. 40: 224-9. PMID: 22260695
9. Swine acute diarrhea syndrome coronavirus replication is reduced by inhibition of the extracellular signal-regulated kinase (ERK) signaling pathway.  |  Zhang, J., et al. 2022. Virology. 565: 96-105. PMID: 34768113
10. Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.  |  Charest, DL., et al. 1993. Mol Cell Biol. 13: 4679-90. PMID: 7687743
11. Mitogen-activated protein kinase phosphatase 1 is overexpressed in prostate cancers and is inversely related to apoptosis.  |  Magi-Galluzzi, C., et al. 1997. Lab Invest. 76: 37-51. PMID: 9010448
12. Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translocation.  |  Khokhlatchev, AV., et al. 1998. Cell. 93: 605-15. PMID: 9604935

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERK 1 Antibody (E-6) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-ERK 1 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERK 1 Antibody (E-6) Specifications

Alternate Product Name: MAPK3

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 291-335

Epitope Target: ERK 1

Epitope Species: rat

Product Reactivity/Detection: ERK 1 p44

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for ERK 1 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p-ERK antibody is p-ERK Antibody (E-4): sc-7383.