p-ERK Antibody (E-4): sc-7383

p-ERK Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects ERK phosphorylated at Tyr 204 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-ERK monoclonal antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERK proteins, which include ERK 1 and ERK 2, play a crucial role in mediating signal transduction pathways triggered by growth factors, hormones, and neurotransmitters. ERK proteins are located in the cytoplasm and translocate to the nucleus upon activation, where ERK proteins regulate gene expression and influence cell proliferation, differentiation, and survival. Dual phosphorylation of ERK at specific threonine and tyrosine residues is essential for full enzymatic activation, allowing phosphorylation of downstream targets on serine and threonine residues. Key upstream regulators of ERK include MAP kinase kinase (MEK), MEK kinase, and Raf-1, which are vital for proper functioning of the MAPK signaling cascade. Additionally, the ERK family comprises three other members: ERK 3, ERK 5, and ERK 6, each contributing to diverse cellular processes. anti-ERK antibody (E-4) is an invaluable resource for investigating ERK phosphorylation at Tyr 204, a critical modification required for activation and role in the MAPK signaling cascade.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-ERK Antibody (E-4) References:

1. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
2. Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product.  |  Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
3. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
4. Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.  |  Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
5. Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).  |  Payne, DM., et al. 1991. EMBO J. 10: 885-92. PMID: 1849075
6. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
7. Components of a new human protein kinase signal transduction pathway.  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
8. ERK6, a mitogen-activated protein kinase involved in C2C12 myoblast differentiation.  |  Lechner, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 4355-9. PMID: 8633070

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-ERK Antibody (E-4) has 3,292 citations in a variety of scientific publications.

Primary antibodies like Anti-p-ERK Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-ERK Antibody (E-4) Specifications

Alternate Product Name: p44/42 MAPK

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: Tyr 204

Epitope Target: ERK

Epitope Species: human

Product Reactivity/Detection: ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3,292 citations in various scientific publications

Available Conjugates/Forms for p-ERK Antibody (E-4): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, TRITC: Rhodamine