EP4 Antibody (C-4): sc-55596

EP4 Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects EP4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EP4 (C-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EP4, a member of the E-prostanoid receptor family, plays a crucial role in mediating prostaglandin E2 effects, a significant lipid mediator involved in various physiological processes, including renal function. EP4 is predominantly expressed in the glomerulus and collecting duct of the kidney, where EP4 couples to Gs proteins to stimulate adenylyl cyclase activity. This action leads to an increase in cyclic adenosine monophosphate (cAMP) levels, which is essential for regulating glomerular tone and influencing renal renin release. Proper functioning of EP4 is vital for maintaining fluid and electrolyte balance, and dysregulation has been implicated in various renal pathologies. Understanding EP4 in kidney physiology can provide insights into potential therapeutic targets for conditions such as hypertension and chronic kidney disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

EP4 Antibody (C-4) References:

1. Prostaglandin E receptors and the kidney.  |  Breyer, MD. and Breyer, RM. 2000. Am J Physiol Renal Physiol. 279: F12-23. PMID: 10894784
2. Selective activation of E-type prostanoid(3)-receptors reduces myocardial infarct size. A novel insight into the cardioprotective effects of prostaglandins.  |  Thiemermann, C. and Zacharowski, K. 2000. Pharmacol Ther. 87: 61-7. PMID: 10924742
3. Expression of prostaglandin E receptor EP4 subtype in rat adrenal zona glomerulosa: involvement in aldosterone release.  |  Muro, S., et al. 2000. Endocr J. 47: 429-36. PMID: 11075723
4. Multiple signal transduction pathways through two prostaglandin E receptor EP3 subtype isoforms expressed in human uterus.  |  Kotani, M., et al. 2000. J Clin Endocrinol Metab. 85: 4315-22. PMID: 11095474
5. G protein-coupled prostanoid receptors and the kidney.  |  Breyer, MD. and Breyer, RM. 2001. Annu Rev Physiol. 63: 579-605. PMID: 11181968
6. A novel prostaglandin E receptor 4 (EP4) small molecule antagonist induces articular cartilage regeneration.  |  Jin, Y., et al. 2022. Cell Discov. 8: 24. PMID: 35256606
7. PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis.  |  Jiang, W., et al. 2022. Bone Res. 10: 27. PMID: 35260562
8. Macrophages evoke autophagy of hepatic stellate cells to promote liver fibrosis in NAFLD mice via the PGE2/EP4 pathway.  |  Cao, Y., et al. 2022. Cell Mol Life Sci. 79: 303. PMID: 35588334
9. PGE2-EP2/EP4 signaling elicits immunosuppression by driving the mregDC-Treg axis in inflammatory tumor microenvironment.  |  Thumkeo, D., et al. 2022. Cell Rep. 39: 110914. PMID: 35675777
10. Complementary roles of EP2 and EP4 receptors in malignant glioma.  |  Qiu, J., et al. 2023. Br J Pharmacol. 180: 2623-2640. PMID: 37232020
11. [Molecular biology of prostaglandin E receptors--expression of multi-function by PGE receptor subtypes and isoforms].  |  Ichikawa, A. 1998. Nihon Rinsho. 56: 1813-8. PMID: 9702058
12. Regulation of renal function by prostaglandin E receptors.  |  Breyer, MD., et al. 1998. Kidney Int Suppl. 67: S88-94. PMID: 9736261

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EP4 Antibody (C-4) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-EP4 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EP4 Antibody (C-4) Specifications

Alternate Product Name: PTGER4

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 329-488

Epitope Target: EP4

Epitope Species: human

Product Reactivity/Detection: EP4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for EP4 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin