DOHH Antibody (E-2): sc-271868
- DOHH Antibody (E-2) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-302 representing full length DOHH of human origin
- Anti-DOHH Antibody (E-2) is recommended for detection of DOHH of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DOHH Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DOHH Antibody (E-2) for WB applications. These reagents are now offered in bundles with DOHH Antibody (E-2) (see ordering information below).
QUICK LINKS
SEE ALSO...
DOHH Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects DOHH in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DOHH (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DOHH, or deoxyhypusine hydroxylase/monooxygenase, also referred to as HLRC1 (HEAT-like (PBS lyase) repeat containing 1), is a metalloenzyme that plays a crucial role in the synthesis of hypusine, a unique amino acid modification essential for the function of eukaryotic translation initiation factor 5A (eIF5A). DOHH is characterized by eight tandem HEAT repeats, which are vital for structural integrity and enzymatic activity. DOHH catalyzes the hydroxylation of a deoxyhypusine-containing intermediate formed by deoxyhypusine synthase, thereby converting it into hypusine and activating eIF5A, which is necessary for cell proliferation and growth. The evolutionary conservation of DOHH, along with interaction with eIF5A and deoxyhypusine synthase, underscores importance in cellular processes. Notably, inhibition of DOHH has been linked to cell cycle arrest at the G1/S boundary, suggesting potential as a target for antitumor therapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DOHH Antibody (E-2) | sc-271868 | 200 µg/ml | $322.00 | |||
DOHH Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-529275 | 200 µg Ab; 10 µg BP | $361.00 | |||
DOHH Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-522084 | 200 µg Ab, 40 µg BP | $361.00 | |||
DOHH Antibody (E-2): m-IgG1 BP-HRP Bundle | sc-543428 | 200 µg Ab; 20 µg BP | $361.00 | |||
DOHH Antibody (E-2) AC | sc-271868 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DOHH Antibody (E-2) HRP | sc-271868 HRP | 200 µg/ml | $322.00 | |||
DOHH Antibody (E-2) FITC | sc-271868 FITC | 200 µg/ml | $336.00 | |||
DOHH Antibody (E-2) PE | sc-271868 PE | 200 µg/ml | $349.00 | |||
DOHH Antibody (E-2) Alexa Fluor® 488 | sc-271868 AF488 | 200 µg/ml | $364.00 | |||
DOHH Antibody (E-2) Alexa Fluor® 546 | sc-271868 AF546 | 200 µg/ml | $364.00 | |||
DOHH Antibody (E-2) Alexa Fluor® 594 | sc-271868 AF594 | 200 µg/ml | $364.00 | |||
DOHH Antibody (E-2) Alexa Fluor® 647 | sc-271868 AF647 | 200 µg/ml | $364.00 | |||
DOHH Antibody (E-2) Alexa Fluor® 680 | sc-271868 AF680 | 200 µg/ml | $364.00 | |||
DOHH Antibody (E-2) Alexa Fluor® 790 | sc-271868 AF790 | 200 µg/ml | $364.00 |