DOHH Antibody (B-12): sc-376929

DOHH Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects the DOHH protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DOHH (B-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DOHH protein, also known as deoxyhypusine hydroxylase or HLRC1 (HEAT-like repeat containing 1), plays a crucial role in the post-translational modification of eukaryotic translation initiation factor 5A (eIF5A) by catalyzing the hydroxylation of deoxyhypusine to form hypusine, a modification essential for eIF5A′s function in cell proliferation and differentiation. The presence of eight tandem HEAT repeats in the DOHH structure is significant as these contribute to DOHH′s stability and interaction with other cellular components, facilitating enzymatic activity. The evolutionary conservation of DOHH, along with deoxyhypusine synthase and eIF5A, underscores DOHH′s importance in cellular processes, and inhibition of DOHH has been linked to cell cycle arrest at the G1/S boundary, suggesting potential as a target for antitumor therapies.

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DOHH Antibody (B-12) References:

1. Horizontal gene transfer and archaeal origin of deoxyhypusine synthase homologous genes in bacteria.  |  Brochier, C., et al. 2004. Gene. 330: 169-76. PMID: 15087136
2. Screening assay for the identification of deoxyhypusine synthase inhibitors.  |  Sommer, MN., et al. 2004. J Biomol Screen. 9: 434-8. PMID: 15296643
3. Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex.  |  Jao, DL. and Chen, KY. 2006. J Cell Biochem. 97: 583-98. PMID: 16215987
4. Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzyme.  |  Park, JH., et al. 2006. Proc Natl Acad Sci U S A. 103: 51-6. PMID: 16371467
5. The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A).  |  Park, MH. 2006. J Biochem. 139: 161-9. PMID: 16452303
6. Deoxyhypusine hydroxylase is a Fe(II)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis [corrected].  |  Kim, YS., et al. 2006. J Biol Chem. 281: 13217-13225. PMID: 16533814
7. Specificity of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction: identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A.  |  Kang, KR., et al. 2007. J Biol Chem. 282: 8300-8. PMID: 17213197
8. Posttranslational synthesis of hypusine: evolutionary progression and specificity of the hypusine modification.  |  Wolff, EC., et al. 2007. Amino Acids. 33: 341-50. PMID: 17476569

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DOHH Antibody (B-12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-DOHH Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DOHH Antibody (B-12) Specifications

Alternate Product Name: DOHH

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 271-299

Epitope Target: DOHH

Epitope Species: human

Product Reactivity/Detection: DOHH

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for DOHH Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred DOHH antibody is DOHH Antibody (E-2): sc-271868.