Derlin-2 Antibody (D-10): sc-398573
- Derlin-2 Antibody (D-10) is a mouse monoclonal IgG2b κ Derlin-2 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 219-236 at the C-terminus of Derlin-2 of human origin
- Derlin-2 Antibody (D-10) is recommended for detection of Derlin-2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Derlin-2 Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Derlin-2 Antibody (D-10) for WB applications. These reagents are now offered in bundles with Derlin-2 Antibody (D-10) (see ordering information below).
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Derlin-2 Antibody (D-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects Derlin-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Derlin-2 (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Derlin-2 plays a critical role in the endoplasmic reticulum-associated degradation (ERAD) pathway, which maintains cellular homeostasis by ensuring misfolded or unassembled proteins are efficiently targeted for degradation. This process is vital because accumulated proteins can lead to cellular stress and contribute to various diseases, including neurodegenerative disorders and cancer. Derlin-2 functions as a multi-pass membrane protein localized to the endoplasmic reticulum, facilitating the translocation of misfolded proteins from the ER to the cytosol for proteasomal degradation. Derlin-2 shows high expression in tissues such as the spleen, pancreas, lung, liver, thymus, and ovary, and Derlin-2 overexpression has been linked to hepatocarcinomas, highlighting a potential role in tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Derlin-2 Antibody (D-10) References:
- Cloning and characterization of F-LANa, upregulated in human liver cancer. | Ying, H., et al. 2001. Biochem Biophys Res Commun. 286: 394-400. PMID: 11500051
- A membrane protein required for dislocation of misfolded proteins from the ER. | Lilley, BN. and Ploegh, HL. 2004. Nature. 429: 834-40. PMID: 15215855
- A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol. | Ye, Y., et al. 2004. Nature. 429: 841-7. PMID: 15215856
- The retrotranslocation protein Derlin-1 binds peptide:N-glycanase to the endoplasmic reticulum. | Katiyar, S., et al. 2005. Mol Biol Cell. 16: 4584-94. PMID: 16055502
- Multiprotein complexes that link dislocation, ubiquitination, and extraction of misfolded proteins from the endoplasmic reticulum membrane. | Lilley, BN. and Ploegh, HL. 2005. Proc Natl Acad Sci U S A. 102: 14296-301. PMID: 16186509
- Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response and are required for ER-associated degradation. | Oda, Y., et al. 2006. J Cell Biol. 172: 383-93. PMID: 16449189
- Signal peptide peptidase is required for dislocation from the endoplasmic reticulum. | Loureiro, J., et al. 2006. Nature. 441: 894-7. PMID: 16738546
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Derlin-2 Antibody (D-10) | sc-398573 | 200 µg/ml | $316.00 | |||
Derlin-2 Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-530848 | 200 µg Ab; 10 µg BP | $354.00 | |||
Derlin-2 Antibody (D-10): m-IgGκ BP-HRP Bundle | sc-524396 | 200 µg Ab, 40 µg BP | $354.00 | |||
Derlin-2 Antibody (D-10) AC | sc-398573 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Derlin-2 Antibody (D-10) HRP | sc-398573 HRP | 200 µg/ml | $316.00 | |||
Derlin-2 Antibody (D-10) FITC | sc-398573 FITC | 200 µg/ml | $330.00 | |||
Derlin-2 Antibody (D-10) PE | sc-398573 PE | 200 µg/ml | $343.00 | |||
Derlin-2 Antibody (D-10) Alexa Fluor® 488 | sc-398573 AF488 | 200 µg/ml | $357.00 | |||
Derlin-2 Antibody (D-10) Alexa Fluor® 546 | sc-398573 AF546 | 200 µg/ml | $357.00 | |||
Derlin-2 Antibody (D-10) Alexa Fluor® 594 | sc-398573 AF594 | 200 µg/ml | $357.00 | |||
Derlin-2 Antibody (D-10) Alexa Fluor® 647 | sc-398573 AF647 | 200 µg/ml | $357.00 | |||
Derlin-2 Antibody (D-10) Alexa Fluor® 680 | sc-398573 AF680 | 200 µg/ml | $357.00 | |||
Derlin-2 Antibody (D-10) Alexa Fluor® 790 | sc-398573 AF790 | 200 µg/ml | $357.00 | |||
Derlin-2 (D-10) Neutralizing Peptide | sc-398573 P | 100 µg/0.5 ml | $68.00 |