Cytokeratin 10/13 Antibody (DE-K13) recommended for detection of Cytokeratin 10 and Cytokeratin 13 of human origin by WB, IP, IF and IHC(P)

Cytokeratin 10/13 Antibody (DE-K13): sc-6258

Cytokeratin 10/13 Antibody (DE-K13) is rated 5.0 out of 5 by 5.
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Datasheets

    • Cytokeratin 10/13 Antibody (DE-K13) is a mouse monoclonal IgG2a κ, cited in 10 publications, provided at 200 µg/ml
    • raised against Cytoskeletal preparation extracted from human ectocervical epithelium
    • recommended for detection of Cytokeratin 10 and Cytokeratin 13 of human origin by WB, IP, IF and IHC(P); specific for Cytokeratin 13 by IHC(P)
    • See Cytokeratin 10 (LH2): sc-53252 for Cytokeratin 10/13 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can Cytokeratin 10/13 (DE-K13): sc-6258 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, Cytokeratin 10/13 (DE-K13): sc-6258 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Useful as a control antibody to study protein Useful as a control antibody to study protein-protein interactions in Armstrong, SM. et al. 2012. -SCBT Publication Review
Date published: 2015-03-17
Rated 5 out of 5 by from In In-house data shows strong banding in human samples. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by from Howie Howie, HL. et al. 2011 used to detect Cytokeratin 10/13 in human cell lines using WB. -SCBT Publication Review
Date published: 2015-02-15
Rated 5 out of 5 by from Distinctive cytoskeletal staining using paraffin Distinctive cytoskeletal staining using paraffin-embedded human samples. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by from IF data shows cytoskeletal protein IF data shows cytoskeletal protein. -SCBT QC
Date published: 2015-01-17
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