cystatin C Antibody (T12): sc-73879

Cystatin C Antibody (T12) is a rat monoclonal IgG2b antibody that detects cystatin C in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. Anti-cystatin C antibody (T12) recognizes a crucial cysteine protease inhibitor that plays a significant role in regulating proteolytic activity in extracellular environments, making cystatin C vital for maintaining cellular homeostasis and preventing unwanted tissue damage. Cystatin C is predominantly secreted and forms a two-fold symmetric dimer, which is essential for modulating cysteine protease activity and influencing the expression of class II major histocompatibility complex molecules. Cystatin C serves as a reliable biomarker for kidney function and glomerular filtration rate, with expression levels providing insights into renal health. Mutations in the cystatin C gene can lead to protein aggregates, which are associated with hereditary cerebral amyloid angiopathy and increased risk of cerebral hemorrhage. While both wild-type and mutant forms of cystatin C can form inactive dimers in a concentration-dependent manner, the mutant variant dimerizes at lower concentrations and shows greater vulnerability to degradation by serine proteases, potentially promoting aggregation. In neuronal cells, oxidative stress enhances cystatin C expression, suggesting a role in apoptosis regulation and highlighting cystatin C′s importance in various physiological and pathological processes.

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cystatin C Antibody (T12) References:

1. Analytical study of three cystatin C assays and their impact on cystatin C-based GFR-prediction equations.  |  Delanaye, P., et al. 2008. Clin Chim Acta. 398: 118-24. PMID: 18805407
2. Cystatin C and cathepsin B production by alveolar macrophages from smokers and nonsmokers.  |  Warfel, AH., et al. 1991. J Leukoc Biol. 49: 41-7. PMID: 1984484
3. Investigation of cystatin C and cystatin C based estimated glomerular filtration rate in pregnant patients with heart failure.  |  Xu, M., et al. 2012. Clin Chem Lab Med. 50: 1125-8. PMID: 22706257
4. Reference intervals for serum cystatin C and factors influencing cystatin C levels other than renal function in the elderly.  |  Wei, L., et al. 2014. PLoS One. 9: e86066. PMID: 24465871
5. Creatinine Versus Cystatin C for Estimating GFR in Patients With Liver Cirrhosis.  |  Torre, A., et al. 2016. Am J Kidney Dis. 67: 342-4. PMID: 26530874
6. Autophagy dysfunction and regulatory cystatin C in macrophage death of atherosclerosis.  |  Li, W., et al. 2016. J Cell Mol Med. 20: 1664-72. PMID: 27079462
7. Lack of association between cystatin C and different coronary atherosclerotic manifestations.  |  Larsen, TR., et al. 2017. Scand J Clin Lab Invest. 77: 574-581. PMID: 28749732
8. Evaluation of cystatin C in malignancy and comparability of estimates of GFR in oncology patients.  |  Jones, M., et al. 2017. Pract Lab Med. 8: 95-104. PMID: 28856234
9. Relationships and Clinical Utility of Creatinine, Cystatin C, eGFRs, GFRs, and Clearances.  |  Toffaletti, JG. 2017. J Appl Lab Med. 2: 413-422. PMID: 33636846
10. Cystatin C and the difference between cystatin C and serum creatinine: Improved metrics to predict waitlist mortality among patients with decompensated cirrhosis.  |  Cullaro, G., et al. 2025. Liver Transpl. 31: 24-31. PMID: 39041923

The preferred cystatin C antibody is cystatin C Antibody (A-10): sc-515732.