cystatin C Antibody (A-10): sc-515732

cystatin C Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects cystatin C protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-cystatin C antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cystatin C is a crucial cysteine protease inhibitor that plays a significant role in regulating proteolytic activity in extracellular environments, thereby maintaining cellular homeostasis and preventing unwanted tissue damage. cystatin C is primarily secreted by various cell types, including epithelial and endothelial cells, and expression is closely linked to kidney function and glomerular filtration rate, making cystatin C a valuable biomarker for renal health. Notably, cystatin C forms a two-fold symmetric dimer, which is essential for function in modulating cysteine protease activity and influencing the expression of class II major histocompatibility complex molecules. Mutations in the cystatin C gene can lead to the formation of protein aggregates, which are associated with hereditary cystatin C amyloid angiopathy and cerebral hemorrhage. In neuronal cells, oxidative stress can induce cystatin C expression, which may have implications for apoptosis regulation. cystatin C (A-10) antibody′s versatility in various applications makes this monoclonal antibody an indispensable tool for researchers studying protease inhibition, kidney function, and related pathologies.

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cystatin C Antibody (A-10) References:

1. Involvement of cystatin C in oxidative stress-induced apoptosis of cultured rat CNS neurons.  |  Nishio, C., et al. 2000. Brain Res. 873: 252-62. PMID: 10930551
2. Human cystatin C, an amyloidogenic protein, dimerizes through three-dimensional domain swapping.  |  Janowski, R., et al. 2001. Nat Struct Biol. 8: 316-20. PMID: 11276250
3. Distinct properties of wild-type and the amyloidogenic human cystatin C variant of hereditary cerebral hemorrhage with amyloidosis, Icelandic type.  |  Calero, M., et al. 2001. J Neurochem. 77: 628-37. PMID: 11299325
4. Bm-CPI-2, a cystatin homolog secreted by the filarial parasite Brugia malayi, inhibits class II MHC-restricted antigen processing.  |  Manoury, B., et al. 2001. Curr Biol. 11: 447-51. PMID: 11301256
5. Cystatin C is an independent predictor of fasting and post-methionine load total homocysteine concentrations among stable renal transplant recipients.  |  Aras O,., et al. 2001. Clin Chem. 47: 1263-8. PMID: 11427458
6. Cystatins.  |  Abrahamson, M., et al. 2003. Biochem Soc Symp. 179-99. PMID: 14587292
7. Clinical usefulness of serum cystatin C and the pertinent estimation of glomerular filtration rate based on cystatin C.  |  Cha, RH., et al. 2010. Nephrology (Carlton). 15: 768-76. PMID: 21175963
8. Proteolysis of cystatin C by cathepsin D in the breast cancer microenvironment.  |  Laurent-Matha, V., et al. 2012. FASEB J. 26: 5172-81. PMID: 22898924
9. Urinary cystatin C and tubular proteinuria predict progression of diabetic nephropathy.  |  Kim, SS., et al. 2013. Diabetes Care. 36: 656-61. PMID: 23093662
10. Cystatin superfamily. Evidence that family II cystatin genes are evolutionarily related to family III cystatin genes.  |  Saitoh, E., et al. 1988. Biol Chem Hoppe Seyler. 369 Suppl: 191-7. PMID: 3202964
11. eGFR calculated from cystatin C: implications for dosing of direct oral anticoagulants.  |  Shin, JI., et al. 2024. Nephrol Dial Transplant. 39: 2117-2119. PMID: 39030050
12. Modification of cystatin C activity by bacterial proteinases and neutrophil elastase in periodontitis.  |  Abrahamson, M., et al. 1997. Mol Pathol. 50: 291-7. PMID: 9536278

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cystatin C Antibody (A-10) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-cystatin C Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cystatin C Antibody (A-10) Specifications

Alternate Product Name: cystatin C

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 26-87

Epitope Target: cystatin C

Epitope Species: human

Product Reactivity/Detection: cystatin C

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for cystatin C Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin