CUL-3 Antibody (H-1) recommended for detection of CUL-3 of mouse, rat and human origin by WB, IP, IF and ELISA

CUL-3 Antibody (H-1): sc-166053

CUL-3 Antibody (H-1) is rated 5.0 out of 5 by 2.
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Datasheets

    • CUL-3 Antibody (H-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-293 of CUL-3 (cullin-3) of human origin
    • recommended for detection of CUL-3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Could this antibody detect cul3-nedd8?

Asked by: mmabb
Thank you for your question. The exact epitope of this antibody has not been mapped and we do not have any information regarding reactivity to nedd8. It is made against a recombinant protein mapping to amino acids 1-239 of human Cullin-3, accession#Q13618. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-04

Are there any issues with using sc-166053: CUL-3 (H-1) mouse monoclonal primary antibody on mouse samples?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-166053 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Strong band in WB in HeLa Strong band in WB in HeLa, K-562, Jurkat, Ramos, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Positive signal in WB in human transfected Positive signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-11-26
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