CUL-3 Antibody (G-8) recommended for detection of CUL-3 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

CUL-3 Antibody (G-8): sc-166110

CUL-3 Antibody (G-8) is rated 4.7 out of 5 by 9.
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Datasheets

    • Anti-CUL-3 Antibody (G-8) is a mouse monoclonal IgG2b CUL-3 antibody provided at 200 µg/ml
    • raised against amino acids 1-293 of CUL-3 (cullin-3) of human origin
    • CUL-3 Antibody (G-8) is recommended for detection of CUL-3 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine and avian
    • Anti-CUL-3 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CUL-3 (G-8): sc-166110.
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I am using CUL-3 (G-8): sc-166110 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works good in kidney homogenates We have bought this antibody for analyzing cullins in kidney homogenates using western blot. A specific band between 75-100 kDa was observed. Concentration 1:250 in BSA.
Date published: 2019-11-22
Rated 4 out of 5 by from works well in WB the strongest band is the right size for Cul3, but there are some weak non specific bands and signal to noise could be better.
Date published: 2018-05-17
Rated 5 out of 5 by from Good antibody This antibody works well without any nonspecific background in western blotting experiments of hepatocarcinoma HepG2 cell line
Date published: 2017-05-12
Rated 5 out of 5 by from Excellent product The CUL-3 Antibody (G-8): sc-166110 works very well for Western blot, showing strong band with minimal background.
Date published: 2017-02-03
Rated 5 out of 5 by from 效果不错~ 大概半个月前申请的cul-3抗体小样,最近做了western blot,可以检测到目的条带,效果还不错~!!!
Date published: 2017-01-09
Rated 4 out of 5 by from good in western work well in western in Jurkat cells, week in IP in Jurkat
Date published: 2016-11-14
Rated 5 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-17
Rated 5 out of 5 by from Strong band in WB in HeLa Strong band in WB in HeLa, K-562, Jurkat, Ramos and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-05-03
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