CS1 Antibody (162.1): sc-53577

CS1 Antibody (162.1) is a mouse monoclonal IgG2b kappa light chain antibody that detects CS1 protein in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CS1 (162.1) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CS1, also known as novel Ly9, SLAMF7, 19A24, or CRACC, is a homophilic cell surface receptor that plays a crucial role in immune responses. CS1 belongs to the signaling lymphocytic activation molecule (SLAM) family, predominantly expressed on natural killer (NK) cells, T cells, and activated B cells. CS1 features immunoreceptor tyrosine-based switch motifs in its cytoplasmic domain, which are essential for signaling capabilities. Unlike other SLAM receptors, CS1 does not recruit SLAM-associated protein (SAP), leading to distinct signaling pathways. In humans, CS1 activates NK cells through an EAT-2-mediated pathway independent of SAP, highlighting its importance in immune regulation. The interaction between CS1 and EAT-2 is critical, as EAT-2 induces CS1 phosphorylation, which subsequently activates downstream cytotoxicity effectors such as phospholipase C gamma and phosphoinositide 3-kinase upon ligand binding. In mice, the association of EAT-2 with CS1 has an inhibitory effect on NK cell activation, illustrating the complex regulatory mechanisms involved in immune responses.

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CS1 Antibody (162.1) References:

1. Mouse novel Ly9: a new member of the expanding CD150 (SLAM) family of leukocyte cell-surface receptors.  |  Tovar, V., et al. 2002. Immunogenetics. 54: 394-402. PMID: 12242590
2. Molecular and functional characterization of a CS1 (CRACC) splice variant expressed in human NK cells that does not contain immunoreceptor tyrosine-based switch motifs.  |  Lee, JK., et al. 2004. Eur J Immunol. 34: 2791-9. PMID: 15368295
3. The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells.  |  Tassi, I. and Colonna, M. 2005. J Immunol. 175: 7996-8002. PMID: 16339536
4. Molecular analysis of NTB-A signaling: a role for EAT-2 in NTB-A-mediated activation of human NK cells.  |  Eissmann, P. and Watzl, C. 2006. J Immunol. 177: 3170-7. PMID: 16920955
5. CS1 (SLAMF7) inhibits production of proinflammatory cytokines by activated monocytes.  |  Kim, JR., et al. 2013. Inflamm Res. 62: 765-72. PMID: 23695528
6. Systematically optimized BCMA/CS1 bispecific CAR-T cells robustly control heterogeneous multiple myeloma.  |  Zah, E., et al. 2020. Nat Commun. 11: 2283. PMID: 32385241
7. CS1 Expression Pattern in NK Cells and Correlated Factors in Plasma Cell dyscrasias: Implications for Elotuzumab Therapy and CAR-T Efficacy.  |  Li, C., et al. 2024. J Cancer. 15: 3065-3075. PMID: 38706917
8. A novel receptor involved in T-cell activation.  |  Cocks, BG., et al. 1995. Nature. 376: 260-3. PMID: 7617038
9. SLAM and its role in T cell activation and Th cell responses.  |  Aversa, G., et al. 1997. Immunol Cell Biol. 75: 202-5. PMID: 9107577
10. Engagement of the signaling lymphocytic activation molecule (SLAM) on activated T cells results in IL-2-independent, cyclosporin A-sensitive T cell proliferation and IFN-gamma production.  |  Aversa, G., et al. 1997. J Immunol. 158: 4036-44. PMID: 9126961
11. The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.  |  Sayos, J., et al. 1998. Nature. 395: 462-9. PMID: 9774102
12. Effector pathways regulating T cell activation.  |  Favero, J. and Lafont, V. 1998. Biochem Pharmacol. 56: 1539-47. PMID: 9973174

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CS1 Antibody (162.1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CS1 Antibody (162.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CS1 Antibody (162.1) Specifications

Alternate Product Name: CS1

Product Clone Name: 162.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: CS1

Epitope Species: human

Product Reactivity/Detection: CS1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CS1 Antibody (162.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin