CROT Antibody (E-2): sc-365408

CROT Antibody (E-2) is a mouse monoclonal IgG1 antibody that detects CROT (carnitine O-octanoyltransferase) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CROT is a vital enzyme located in peroxisomes, primarily expressed in the liver, kidney, and proximal intestinal epithelium, where CROT plays a crucial role in lipid metabolism. By catalyzing the reversible conversion of acyl-CoAs to their corresponding carnitine esters, CROT facilitates the transport of fatty acids from peroxisomes to mitochondria, enabling their further degradation through β-oxidation. This process is essential for energy metabolism in eukaryotic cells, particularly for medium-chain fatty acids, which are critical energy sources. Notably, CROT exhibits a preference for straight and branched medium-chain acyl-CoAs with chain lengths ranging from C6 to C10, underscoring CROT′s importance in metabolic pathways. Additionally, malonyl-CoA can inhibit CROT activity, highlighting the regulatory mechanisms that control fatty acid oxidation and energy production. Anti-CROT antibody (E-2) serves as an invaluable tool for researchers studying lipid metabolism and related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CROT Antibody (E-2) References:

1. Single Cell Gene Co-Expression Network Reveals FECH/CROT Signature as a Prognostic Marker.  |  Chen, X., et al. 2019. Cells. 8: PMID: 31295943
2. Factors Associated With Complete Remission After Rituximab Therapy for Pemphigus.  |  Kushner, CJ., et al. 2019. JAMA Dermatol. 155: 1404-1409. PMID: 31642878
3. LncRNA EGOT regulates the proliferation and apoptosis of colorectal cancer by miR-33b-5p/CROT axis.  |  Ni, Y., et al. 2020. Biosci Rep.. PMID: 32373939
4. CROT (Carnitine O-Octanoyltransferase) Is a Novel Contributing Factor in Vascular Calcification via Promoting Fatty Acid Metabolism and Mitochondrial Dysfunction.  |  Okui, T., et al. 2021. Arterioscler Thromb Vasc Biol. 41: 755-768. PMID: 33356393
5. Acox2 is a regulator of lysine crotonylation that mediates hepatic metabolic homeostasis in mice.  |  Zhang, Y., et al. 2022. Cell Death Dis. 13: 279. PMID: 35351852
6. The Regulators of Peroxisomal Acyl-Carnitine Shuttle CROT and CRAT Promote Metastasis in Melanoma.  |  Lasheras-Otero, I., et al. 2023. J Invest Dermatol. 143: 305-316.e5. PMID: 36058299
7. The miR-33a-5p/CROT axis mediates ovarian cancer cell behaviors and chemoresistance via the regulation of the TGF-β signal pathway.  |  Li, X., et al. 2022. Front Endocrinol (Lausanne). 13: 950345. PMID: 36120434
8. Carnitine o-octanoyltransferase is a p53 target that promotes oxidative metabolism and cell survival following nutrient starvation.  |  Sanford, JD., et al. 2023. J Biol Chem. 299: 104908. PMID: 37307919
9. Continuous-flow electrorotation (cROT): improved throughput characterization for dielectric properties of cancer cells.  |  Yoda, K., et al. 2023. Lab Chip. 23: 4986-4996. PMID: 37889126
10. Carnitine O-octanoyltransferase (CROT) deficiency in mice leads to an increase of omega-3 fatty acids.  |  Okui, T., et al. 2024. Front Mol Biosci. 11: 1374316. PMID: 39076376

The preferred CROT antibody is CROT Antibody (H-1): sc-365976.