CROT Antibody (H-1): sc-365976

CROT Antibody (H-1) is a mouse monoclonal IgM antibody that detects CROT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-CROT antibody (H-1) is available as the non-conjugated form. CROT, or carnitine O-octanoyltransferase, is a vital enzyme within the carnitine/choline acetyltransferase protein family, which also includes other key enzymes such as carnitine acetyltransferase and carnitine palmitoyltransferase. CROT is primarily located in peroxisomes and is abundantly expressed in the liver, kidney, and proximal intestinal epithelium, where CROT plays a crucial role in lipid metabolism. CROT catalyzes the reversible conversion of acyl-CoAs to their corresponding carnitine esters, a critical step that facilitates the transport of fatty acids from peroxisomes to mitochondria for further degradation. This process is essential for energy metabolism in eukaryotic cells, particularly for the β-oxidation of fatty acids, which is necessary for generating ATP. Notably, CROT exhibits a preference for medium-chain acyl-CoAs, specifically those with chain lengths ranging from C6 to C10, and CROT activity can be inhibited by malonyl-CoA, highlighting the regulatory mechanisms that control fatty acid metabolism.

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CROT Antibody (H-1) References:

1. Molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids.  |  Ferdinandusse, S., et al. 1999. Biochem Biophys Res Commun. 263: 213-8. PMID: 10486279
2. Genomics of the human carnitine acyltransferase genes.  |  van der Leij, FR., et al. 2000. Mol Genet Metab. 71: 139-53. PMID: 11001805
3. Long- and medium-chain fatty acid oxidation is increased in exercise-trained human skeletal muscle.  |  Jong-Yeon, K., et al. 2002. Metabolism. 51: 460-4. PMID: 11912554
4. Redesign of carnitine acetyltransferase specificity by protein engineering.  |  Cordente, AG., et al. 2004. J Biol Chem. 279: 33899-908. PMID: 15155769
5. Structure and function of carnitine acyltransferases.  |  Jogl, G., et al. 2004. Ann N Y Acad Sci. 1033: 17-29. PMID: 15591000
6. Mutagenesis of specific amino acids converts carnitine acetyltransferase into carnitine palmitoyltransferase.  |  Cordente, AG., et al. 2006. Biochemistry. 45: 6133-41. PMID: 16681386
7. Peroxisomes, Refsum's disease and the alpha- and omega-oxidation of phytanic acid.  |  Wanders, RJ. and Komen, JC. 2007. Biochem Soc Trans. 35: 865-9. PMID: 17956234
8. Tacrine-induced liver damage: an analysis of 19 candidate genes.  |  Alfirevic, A., et al. 2007. Pharmacogenet Genomics. 17: 1091-100. PMID: 18004213
9. Short- and medium-chain carnitine acyltransferases and acyl-CoA thioesterases in mouse provide complementary systems for transport of beta-oxidation products out of peroxisomes.  |  Westin, MA., et al. 2008. Cell Mol Life Sci. 65: 982-90. PMID: 18264800
10. MicroRNA modulation of cholesterol homeostasis.  |  Fernández-Hernando, C. and Moore, KJ. 2011. Arterioscler Thromb Vasc Biol. 31: 2378-82. PMID: 22011750
11. miR‑126a‑5p‑Dbp and miR‑31a‑Crot/Mrpl4 interaction pairs crucial for the development of hypertension and stroke.  |  Zhao, Q., et al. 2019. Mol Med Rep. 20: 4151-4167. PMID: 31545431
12. A multi-patient analysis of the center of rotation trajectories using finite element models of the human mandible.  |  Gholamalizadeh, T., et al. 2021. PLoS One. 16: e0259794. PMID: 34780529

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CROT Antibody (H-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CROT Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CROT Antibody (H-1) Specifications

Alternate Product Name: CROT

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 1-300

Epitope Target: CROT

Epitope Species: human

Product Reactivity/Detection: CROT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CROT Antibody (H-1): N/A