CREST Antibody (G-8): sc-515871

CREST Antibody (G-8) is a mouse monoclonal IgG1 antibody that detects CREST in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CREST, or calcium-responsive transactivator, plays a crucial role in neuronal development by localizing to nuclear bodies and facilitating the normal formation of dendritic trees in neurons. CREST features a multifunctional domain that is essential for transcriptional activation, targeting to nuclear bodies, and dimerization, which are vital processes for regulating gene expression in response to calcium signaling. Notably, CREST interacts with the cAMP response element-binding protein (CREB)-binding protein (CBP), a key player in neuronal morphogenesis, underscoring CREST′s importance in the intricate network of signaling pathways that govern neuronal growth and differentiation. While CREST is expressed ubiquitously, CREST levels are notably lower in the spleen, indicating a specialized role in the nervous system. Mice with a targeted disruption of the Ss18l1 (CREST) gene remain viable, yet exhibit significant defects in the development of cortical and hippocampal dendrites, highlighting CREST′s essential function in calcium-dependent dendritic growth and the overall architecture of the nervous system.

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CREST Antibody (G-8) References:

1. Functional domains of paired-like homeoprotein Cart1 and the relationship between dimerization and transcription activity.  |  Furukawa, K., et al. 2002. Genes Cells. 7: 1135-47. PMID: 12390248
2. The OAR/aristaless domain of the homeodomain protein Cart1 has an attenuating role in vivo.  |  Brouwer, A., et al. 2003. Mech Dev. 120: 241-52. PMID: 12559496
3. P300/CBP acts as a coactivator to cartilage homeoprotein-1 (Cart1), paired-like homeoprotein, through acetylation of the conserved lysine residue adjacent to the homeodomain.  |  Iioka, T., et al. 2003. J Bone Miner Res. 18: 1419-29. PMID: 12929931
4. Dendrite development regulated by CREST, a calcium-regulated transcriptional activator.  |  Aizawa, H., et al. 2004. Science. 303: 197-202. PMID: 14716005
5. A multifunctional domain of the calcium-responsive transactivator (CREST) that inhibits dendritic growth in cultured neurons.  |  Pradhan, A. and Liu, Y. 2005. J Biol Chem. 280: 24738-43. PMID: 15866867
6. Calcium-responsive transactivator (CREST) protein shares a set of structural and functional traits with other proteins associated with amyotrophic lateral sclerosis.  |  Kukharsky, MS., et al. 2015. Mol Neurodegener. 10: 20. PMID: 25888396
7. CREST in the Nucleus Accumbens Core Regulates Cocaine Conditioned Place Preference, Cocaine-Seeking Behavior, and Synaptic Plasticity.  |  Alaghband, Y., et al. 2018. J Neurosci. 38: 9514-9526. PMID: 30228227
8. Prenatal folic acid treatment suppresses acrania and meroanencephaly in mice mutant for the Cart1 homeobox gene.  |  Zhao, Q., et al. 1996. Nat Genet. 13: 275-83. PMID: 8673125
9. Human CART1, a paired-class homeodomain protein, activates transcription through palindromic binding sites.  |  Cai, RL. 1998. Biochem Biophys Res Commun. 250: 305-11. PMID: 9753625
10. Physical and genetic interactions between Alx4 and Cart1.  |  Qu, S., et al. 1999. Development. 126: 359-69. PMID: 9847249

The preferred CREST antibody is CREST Antibody (D-7): sc-515827.