Anti-CREM Antibody (A-2) recommended for detection of CREM of mouse and rat origin by WB, IP, IF and ELISA

Anti-CREM Antibody (A-2): sc-377496

Anti-CREM Antibody (A-2) is rated 5.0 out of 5 by 1.
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Datasheets

    • CREM Antibody (A-2) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against the full length polyhistidine tagged CREM fusion protein fusion protein of mouse origin
    • recommended for detection of CREM of mouse and rat origin by WB, IP, IF and ELISA
    • TransCruz reagent for ChIP application (sc-377496 X, 200 µg/0.1 ml)
    • See CREM (C-2): sc-390426 for CREM antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CREM Antibody (A-2) for WB applications. These reagents are now offered in bundles with CREM Antibody (A-2) (see ordering information below).
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Do you think it will work for detecting human CREM? Thanks!

Asked by: Sheryss
Thank you for your question. This antibody has not yet been tested in human samples and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-12-16

How much sample do you recommend loading on the gel for Western blot detection using CREM (A-2): sc-377496?

Asked by: cjMara
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Good Western blot data of CREM expression Good Western blot data of CREM expression in KNRK nuclear extract. -SCBT QC
Date published: 2014-03-30
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