CREB1 Antibody (D-12): sc-377154

CREB-1 Antibody (D-12) is a mouse monoclonal IgG2a kappa light chain antibody engineered to target amino acids 254-327 of human CREB-1, providing reliable detection of CREB-1A, CREB-1B, CREM, and ATF-1 isoforms across mouse, rat, human, and avian species, as well as additional species including equine, canine, bovine, and porcine, through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. CREB-1, also called cAMP response element-binding protein 1, CREB, and CREB1, is a crucial transcription factor located primarily in the cell nucleus, where CREB-1 binds to cAMP response elements (CRE) in the promoters of target genes, thereby regulating essential cellular processes such as metabolism, neuronal plasticity, and cell survival. The functionality of CREB-1 is intricately modulated through interactions with several key proteins, including CREB-binding protein (CBP), which acts as a coactivator facilitating the assembly of the basal transcription complex upon CREB-1 phosphorylation at Serine 133 by protein kinase A. Additionally, CREB-1 interacts with the nuclear coactivator PGC-1α, playing a significant role in neoglucogenesis and energy metabolism, as well as with other regulatory proteins such as TORC (transducers of regulated CREB activity) that enhance transcriptional activity. These protein-protein interactions are essential for the precise regulation of gene expression in response to hormonal and stress signals, highlighting CREB-1′s involvement in critical pathways related to cellular adaptation and function. Furthermore, the dynamic interactions of CREB-1 with binding partners underscore CREB-1′s importance in the pathogenesis of type II diabetes and dilated cardiomyopathy, where dysregulation of these interactions can lead to metabolic and cardiovascular dysfunctions. CREB-1 (D-12) monoclonal antibody is available in both non-conjugated form and various conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CREB1 Antibody (D-12) References:

1. CREB regulates hepatic gluconeogenesis through the coactivator PGC-1.  |  Herzig, S., et al. 2001. Nature. 413: 179-83. PMID: 11557984
2. Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells.  |  Gee, K., et al. 2006. J Biol Chem. 281: 31647-58. PMID: 16920714
3. Expression and regulated nuclear transport of transducers of regulated CREB 1 in retinal ganglion cells.  |  Deng, J., et al. 2009. Neuroscience. 159: 1023-31. PMID: 19356686
4. Assignment of the human gene for CREB1 to chromosome 2q32.3-q34.  |  Taylor, AK., et al. 1990. Genomics. 7: 416-21. PMID: 2142119
5. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
6. Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
7. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
8. Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.  |  Yamamoto, KK., et al. 1988. Nature. 334: 494-8. PMID: 2900470
9. Nuclear protein CBP is a coactivator for the transcription factor CREB.  |  Kwok, RP., et al. 1994. Nature. 370: 223-6. PMID: 7913207
10. Activation of cAMP and mitogen responsive genes relies on a common nuclear factor.  |  Arias, J., et al. 1994. Nature. 370: 226-9. PMID: 8028671
11. Dilated cardiomyopathy in transgenic mice expressing a dominant-negative CREB transcription factor in the heart.  |  Fentzke, RC., et al. 1998. J Clin Invest. 101: 2415-26. PMID: 9616213

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CREB1 Antibody (D-12) has 62 citations in a variety of scientific publications.

Primary antibodies like Anti-CREB1 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CREB1 Antibody (D-12) Specifications

Alternate Product Name: CREB

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 254-327

Epitope Target: CREB-1

Epitope Species: human

Product Reactivity/Detection: CREB-1A, CREB-1B, CREM and ATF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 62 citations in various scientific publications

Available Conjugates/Forms for CREB1 Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent