Anti-CREB-1 Antibody (D-12) recommended for detection of CREB-1A, CREB-1B, CREM and ATF-1 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

Anti-CREB-1 Antibody (D-12): sc-377154

Anti-CREB-1 Antibody (D-12) is rated 4.3 out of 5 by 23.
  • y_2021, m_10, d_24, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_23
  • loc_en_US, sid_377154, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 116ms
  • REVIEWS, PRODUCT
loading
5
14
4
6
3
1
2
1
1
1

Datasheets

    • Anti-CREB-1 Antibody (D-12) is a mouse monoclonal IgG2a κ CREB-1 antibody, cited in 41 publications, provided at 200 µg/ml
    • raised against amino acids 254-327 of CREB-1 of human origin
    • CREB-1 Antibody (D-12) is recommended for detection of CREB-1A, CREB-1B, CREM and ATF-1 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-CREB-1 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-377154 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CREB-1 (D-12): sc-377154.
    • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CREB-1 Antibody (D-12) for WB and IHC(P) applications. These reagents are now offered in bundles with CREB-1 Antibody (D-12) (see ordering information below).
loading

Is this antibody specific for the unphosphorylated form? Or does it grab all CREB-1 (unphosphorylated AND phosphorylated at Ser-133)?

Asked by: bjw23
This antibody is not phospho-specific. It should be able to detect both the phosphorylated and unphosphorylated forms of CREB-1.
Answered by: Technical Support
Date published: 2018-12-21

I would be interested in FREE 10 µg sample of CREB-1 (D-12): sc-377154. Can you provide concentrated one (CREB-1 Antibody (D-12) X: sc-377154 X)?

Asked by: Shogo
We do not offer sample sizes of the concentrated "X" products. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-10

When should the TransCruz reagent sc-377154 X be used instead of the standard form sc-377154?

Asked by: jenniferc
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 8
Date published: 2017-01-10
  • y_2021, m_10, d_24, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_377154, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 82ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from WESTERN BLOT Wstern Blot of mice dorsal hippocampus. Good quality.
Date published: 2021-05-12
Rated 4 out of 5 by from ottimo funzionamento in WB, medio in IF Ho testato il campione omaggio da 10 microlitri ottenuto da DBA e allego l'immagine degli esperimenti
Date published: 2019-05-22
Rated 3 out of 5 by from 少し薄い 組織由来のサンプルに使用したため、薄いバンドの検出となってしまった。しかしバンドは確認できるため、濃度を濃くして再度検討を行う。
Date published: 2019-02-13
Rated 4 out of 5 by from The product is good It could be better, but we do the a band with this antibody in HEK293 NP40 lysates when used at 1:500
Date published: 2018-12-12
Rated 4 out of 5 by from Specific for CREB in WB I overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. CREB D-12 was very specific to CREB in WB (dilution 1:200), as it recognized only GFP-CREB protein, but not GFP-ATF1 or GFP-CREM.
Date published: 2018-08-23
Rated 5 out of 5 by from nice antibody I bought it as a sample and the antibody is quite good - nice signal in WB in lung cancer cell lines
Date published: 2017-12-21
Rated 1 out of 5 by from no band at correct length Just tried it. there is no band near 40 kda. only unspecific band at 65 kda
Date published: 2017-12-20
Rated 4 out of 5 by from good results i used this for western and i loaded 30 ug protein and incubate the membrane with 1:400 concentration it gives me a faint bands so i stripped and used higher concentration 1:250 then i got a good bands
Date published: 2017-09-29
  • y_2021, m_10, d_24, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_23
  • loc_en_US, sid_377154, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT