COL2A1 Antibody (M2139) recommended for detection of Collagen Type II of mouse, rat, human and a origin by WB, IP, IF and IHC(P)

COL2A1 Antibody (M2139): sc-52658

COL2A1 Antibody (M2139) is rated 4.9 out of 5 by 10.
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Datasheets

    • Anti-COL2A1 Antibody (M2139) is a mouse monoclonal IgG2b (kappa light chain) COL2A1 antibody provided at 100 µg/ml
    • raised against the collagen II J1 epitope (triple helical position 551-564) of mouse origin
    • recommended for detection of Collagen Type II of mouse, rat, human and a origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of COL2A1 (M2139): sc-52658.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I would like to detection of collagen type 2 in western blot assay. I tried dilution 1:500 in 5% skinmilk. and 2nd antibody used sc-2005 product. but It does not detection. How can I do? my sample orign is human pellet.

Asked by: vtml
Thank you for your question. We recommend starting with a 1:100 primary antibody dilution for Western blot. For further assistance, please contact Technical Service.
Answered by: Technical Support
Date published: 2019-01-18

Is trial pack available for this antibody ?

Asked by: Ghosh
Thank you for the question. COL2A1 Antibody (M2139) is a mouse monoclonal mouse IgG2b κ (kappa light chain) provided at 100 µg/ml. We currently do offer Sample sizes for this product. Please request this with your supplier. However, you can also purchase the product with our 1 year full product warranty if you are planning to use this product in any of the warranted applications and warranted species: recommended for detection of Collagen Type II of mouse, rat, human and a origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine.
Answered by: Technical Support Europe
Date published: 2017-12-03

I would like to do some IF on my mouse samples. Do you have any suggestions on how this can be successful using a mouse monoclonal primary?

Asked by: Cweed
We recommend using a a directly conjugated primary antibodies. Please contact our special orders department for pricing and availability of sc-52658 conjugates.
Answered by: Technical Support 10
Date published: 2017-01-12

I can find the donkey anti-mouse secondary that I am accustomed to using. What do you recommend as a replacement?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-30
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Rated 5 out of 5 by from Excellent for IF It works very well in whole mount immunofluorescence
Date published: 2018-04-17
Rated 4 out of 5 by from works great on western blot We have used this antibody on human skin tissue. It works great on western blot.
Date published: 2017-09-20
Rated 5 out of 5 by from COL2A1 IHC chick embryo, PFA fixed, cryosectioned 2nd Ab Alexa fluor488
Date published: 2017-09-05
Rated 5 out of 5 by from good The antibody is suitable for cellular immunofluorescence. I used it in chondrocytes.
Date published: 2017-07-03
Rated 5 out of 5 by from Well done! We used the COL2A1 (M2139) is a kind of rat monoclonal antibody IgG2b ( 100 ug/ml). Conclusion: can improve the j - 1 epitope collagen II (551-564), triple helix position of rat.
Date published: 2017-02-10
Rated 5 out of 5 by from Great for Western blot Nice, clean strong band by WB. Highly recommended!
Date published: 2017-01-13
Rated 5 out of 5 by from Strong wb Strong western blot data; clear signal, low background
Date published: 2017-01-01
Rated 5 out of 5 by from Good COL2 antibody! This antibody of COL2 really works well, it can get strong and single band at 1:1000-2000, I think it is a ideal antibody for WB!
Date published: 2016-12-19
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