Cdc45 Antibody (E-3): sc-55568
- Cdc45 Antibody (E-3) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of Cdc45 of human origin
- recommended for detection of Cdc45 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Cdc45 Antibody (E-3) for WB applications. This reagent is now offered in a bundle with Cdc45 Antibody (E-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Cdc45 Antibody (E-3) is a mouse monoclonal IgG1 antibody that detects Cdc45 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdc45 plays a crucial role in the initiation of DNA replication, functioning as a vital component of the pre-replicative complex that ensures accurate duplication of the genome during the cell cycle. Proper localization of Cdc45 at the replication fork is essential for recruiting other key proteins, including the MCM helicase complex, which unwinds DNA strands, allowing for the synthesis of new DNA. Disruption of Cdc45 function can lead to replication stress and genomic instability, which are hallmarks of various cancers. Anti-Cdc45 antibody (E-3) provides reliable detection and quantification of Cdc45, facilitating research into cell cycle regulation and cancer biology.
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Cdc45 Antibody (E-3) References:
- Regulation of Cdc45 in the cell cycle and after DNA damage. | Broderick, R. and Nasheuer, HP. 2009. Biochem Soc Trans. 37: 926-30. PMID: 19614620
- Cdc45 limits replicon usage from a low density of preRCs in mammalian cells. | Wong, PG., et al. 2011. PLoS One. 6: e17533. PMID: 21390258
- Myc-driven chromatin accessibility regulates Cdc45 assembly into CMG helicases. | Nepon-Sixt, BS., et al. 2019. Commun Biol. 2: 110. PMID: 30911685
- Further delineation of CDC45-related Meier-Gorlin syndrome with craniosynostosis and review of literature. | Ting, CY., et al. 2020. Eur J Med Genet. 63: 103652. PMID: 30986546
- CDC45 modulates MCM7 expression and inhibits cell proliferation by suppressing the PI3K/AKT pathway in acute myeloid leukemia. | Zhang, R., et al. 2021. Am J Transl Res. 13: 10218-10232. PMID: 34650692
- Systematic pan‑cancer analysis identifies CDC45 as having an oncogenic role in human cancers. | Lu, Y., et al. 2022. Oncol Rep. 48: PMID: 36082823
- DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiation. | Kingsley, G., et al. 2023. Nucleic Acids Res. 51: 9748-9763. PMID: 37638758
- Elevated CDC45 Expression Predicts Poorer Overall Survival Prognoses and Worse Immune Responses for Kidney Renal Clear Cell Carcinoma via Single-Cell and Bulk RNA-Sequencing. | Zhang, X., et al. 2024. Biochem Genet. 62: 1502-1520. PMID: 37642814
- IGF2BP2 promotes glycolysis and hepatocellular carcinoma stemness by stabilizing CDC45 mRNA via m6A modification. | Wu, T., et al. 2023. Cell Cycle. 22: 2245-2263. PMID: 37985379
- Induction of the human CDC45 gene promoter activity by natural compound trans‑resveratrol. | Arakawa, J., et al. 2024. Mol Med Rep. 29: PMID: 38577929
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc45 Antibody (E-3) | sc-55568 | 200 µg/ml | $322.00 | |||
Cdc45 Antibody (E-3): m-IgGκ BP-HRP Bundle | sc-521019 | 200 µg Ab, 40 µg BP | $361.00 |