Cdc42 Antibody (B-9) recommended for detection of Cdc42 of human and ce origin by WB, IP, IF and ELISA

Cdc42 Antibody (B-9): sc-390210

Cdc42 Antibody (B-9) is rated 3.8 out of 5 by 4.
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Datasheets

    • Cdc42 Antibody (B-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a peptide mapping near the C-terminus of Cdc42 of C. elegans origin
    • Anti-Cdc42 Antibody (B-9) is recommended for detection of Cdc42 of human and ce origin by WB, IP, IF and ELISA
    • Anti-Cdc42 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-390210 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc42 (B-9): sc-390210.
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What application is the blocking peptide sc-390210 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Immunofluroscence The antibody stains in a punctuate fashion in human cancer cells.
Date published: 2017-11-10
Rated 4 out of 5 by from Clean bands I got a sample trial antibody and it works great if you use the santacruz antimouse secondary antibody.
Date published: 2017-07-14
Rated 4 out of 5 by from Worked well in WB in 293T whole cell lysates Worked well in WB in 293T whole cell lysates. -SCBT QC
Date published: 2015-03-05
Rated 4 out of 5 by from Strong band in WB in C Strong band in WB in C.elegans whole cell lysate. -SCBT QC
Date published: 2014-11-08
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